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HRD1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: HRD1 Antibody [NBP1-89667] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & endoplasmic reticulum.
Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human lymph node.
Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells
Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human tonsil shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining in human tonsil and skeletal muscle tissues using anti-SYVN1 antibody. Corresponding SYVN1 RNA-seq data are presented ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human cerebral cortex, lymph node, skeletal muscle and tonsil using Anti-SYVN1 antibody NBP1-89667 (A) shows similar ...read more
Immunohistochemistry-Paraffin: HRD1 Antibody [NBP1-89667] - Staining of human cerebral cortex.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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HRD1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PAPGFPFPPPWMGMPLPPPFAFPPMPVPPAGFAGLTPEELRALEGHERQHLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSVNSTEETATTVVAAASSTSIPSSEATTPTPGASPPAPEMERPPAPESVGT
Predicted Species
Mouse (92%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYVN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
HRD1 Protein (NBP1-89667PEP)
Publications
Read Publication using NBP1-89667.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HRD1 Antibody

  • E3 ubiquitin-protein ligase synoviolin
  • EC 6.3.2
  • HRD1EC 6.3.2.-
  • KIAA1810Synovial apoptosis inhibitor 1
  • MGC40372
  • synovial apoptosis inhibitor 1, synoviolin

Background

HRD1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of HRD1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. HRD1 is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. It has been reported that Synoviolin/Hrd1 (expressed in rheumatoid synovium) is a novel causative factor for arthropathy by triggering synovial cell outgrowth through its anti-apoptotic effects. HRD1 contains one ring-type zinc finger.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HRD1 Antibody (NBP1-89667)(1)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for HRD1 Antibody (NBP1-89667) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for HRD1 Antibody (NBP1-89667)

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Blogs on HRD1.

OS9: Taking proteins to the ER finish line
The OS9 protein is a lectin/glycoprotein that maintains endoplasmic reticulum (ER) quality control and ER-associated degradation (the so-called ERAD pathway) of newly synthesized proteins. It is essential for the recognition of terminally misfolded no...  Read full blog post.

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Bioinformatics

Gene Symbol SYVN1