Reactivity | HuSpecies Glossary |
Applications | PAGE |
Concentration | 2 mg/ml |
Dilutions |
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Application Notes | One vial of lysate is sufficient to run 10 lanes (10 ug/lane) of protein.HEK293 cell line lysate (embryonic kidney) |
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Publications |
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Storage | Store at -80C. Avoid freeze-thaw cycles. |
Buffer | Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1x Laemmli sample buffer (62.5 mM Tris-HCl, pH 6.8, 25% Glycerol, 2% SDS, 0.01% Bromophenol blue). Denaturing agents have not been added. |
Concentration | 2 mg/ml |
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