![Immunocytochemistry/Immunofluorescence: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunofluorescence staining of HNF-6/ONECUT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HNF-6/ONECUT1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/HNF-6-ONECUT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99314-img0001.jpg "Immunocytochemistry/Immunofluorescence: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunofluorescence staining of HNF-6/ONECUT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HNF-6/ONECUT1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus .")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human HNF-6/ONECUT1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ONECUT1 |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen and protein A Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ONECUT1 |
![Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunochemical staining HNF-6/ONECUT1 in mouse brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HNF-6-ONECUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-99314-img0004.jpg "Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunochemical staining HNF-6/ONECUT1 in mouse brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunochemical staining HNF-6/ONECUT1 in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HNF-6-ONECUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-99314-img0002.jpg "Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunochemical staining HNF-6/ONECUT1 in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunochemical staining HNF-6/ONECUT1 in human hepatoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HNF-6-ONECUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-99314-img0003.jpg "Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunochemical staining HNF-6/ONECUT1 in human hepatoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")