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HNF-3 alpha/FoxA1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP2-38628] - Staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli fibrillar center.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP2-38628] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
Orthogonal Strategies: Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP2-38628] - Analysis in human prostate and endometrium tissues. Corresponding HNF-3 alpha/FoxA1 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP2-38628] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP2-38628] - Staining of human prostate shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP2-38628] - Staining of human stomach shows moderate nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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HNF-3 alpha/FoxA1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: QTLDHSGATATGGASELKTPASSTAPPISSGPGALASVPASHPAHGLAPHESQLHLKGDPHYSFNHP
Predicted Species
Mouse (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
HNF-3 alpha/FoxA1 Protein (NBP2-38628PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HNF-3 alpha/FoxA1 Antibody

  • forkhead box A1
  • Forkhead box protein A1
  • FoxA1
  • hepatocyte nuclear factor 3, alpha
  • hepatocyte nuclear factor 3-alpha
  • HNF3 alpha
  • HNF-3 alpha
  • HNF-3A
  • HNF-3-alpha
  • HNF3ATCF-3A
  • MGC33105
  • TCF3A
  • Transcription factor 3A

Background

FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FOXA1
Uniprot