![Western Blot: HNF-3 alpha/FoxA1 Antibody (FOXA1/2230R) - Azide and BSA Free [NBP3-08921] - Western Blot Analysis of HeLa cell lysate using HNF-3 alpha/FoxA1 Rabbit Recombinant Monoclonal Antibody (HNF-3 alpha/FoxA1/2230R).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-FOXA1-2230R-Azide-and-BSA-Free-Western-Blot-NBP3-08921-img0004.jpg "Western Blot: HNF-3 alpha/FoxA1 Antibody (FOXA1/2230R) - Azide and BSA Free [NBP3-08921] - Western Blot Analysis of HeLa cell lysate using HNF-3 alpha/FoxA1 Rabbit Recombinant Monoclonal Antibody (HNF-3 alpha/FoxA1/2230R).")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | FOXA1/2230R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07178). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant human HNF-3 alpha/FoxA1 protein fragment (around aa372-472) (exact sequence is proprietary) (Uniprot: P55317) |
| Localization | Nuclear |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | FOXA1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for HNF-3 alpha/FoxA1 Antibody (NBP3-08921)Find related products by research area.
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| Gene Symbol | FOXA1 |
![Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (FOXA1/2230R) - Azide and BSA Free [NBP3-08921] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeled with HNF-3 alpha/FoxA1 Rabbit Recombinant Monoclonal Antibody (HNF-3 alpha/FoxA1/2230R); followed by goat anti- rabbit IgG- CF488. Phalloidin stains the membrane(Red).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-FOXA1-2230R-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-08921-img0002.jpg "Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (FOXA1/2230R) - Azide and BSA Free [NBP3-08921] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeled with HNF-3 alpha/FoxA1 Rabbit Recombinant Monoclonal Antibody (HNF-3 alpha/FoxA1/2230R); followed by goat anti- rabbit IgG- CF488. Phalloidin stains the membrane(Red).")
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