HMGN1/HMG14 [p Ser20, p Ser24] Antibody

HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species.? As such, this reagent is designed as a "universal" reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family.? Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the "HMG-box;" that of the HMGN family, the "nucleosomal binding domain;" and that of the HMGA family, the "AT-hook." The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as "HMG motif proteins."

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Sheep.

We have publications tested in 1 application: IHC-P.

Showing Publication 1 - 1 of 1.

Publication using NBP1-78102	Applications	Species
Regan JK, Kannan PS, Kemp MW et al. Damage-Associated Molecular Pattern and Fetal Membrane Vascular Injury and Collagen Disorganization in Lipopolysaccharide-Induced Intra-amniotic Inflammation in Fetal Sheep Reprod Sci. 2015-07-07 [PMID: 26156854] (IHC-P, Sheep) Details: HMG14 [p Ser20, p Ser24] antibody was used for IHC-P staining on formalin-fixed chorioamnion tissues of fetal sheep and the IHC assay implicated - paraffin sections, microwave-based antigen retrieval in citric acid buffer at pH 6.0, endogenous peroxidase blocking with methyl alcohol/hydrogen peroxide, blocking with 2% serum in PBS, use of primary at 1:50 dilution, detection with anti-rabbit secondary and Vectastain ABC peroxidase kit with staining enhancement using nickel-DAB followed by incubation with Tris-cobalt (Figure 2).	IHC-P	Sheep

Array NBP1-78102

• HMGN1/HMG14 Antibodies
• HMGN1/HMG14 ELISA Kits
• HMGN1/HMG14 Lysate
• HMGN1/HMG14 Proteins
• HMGN1/HMG14 Proteins and Enzyme