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HMGCL Antibody (S08-7H5)

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Western Blot: HMGCL Antibody (S08-7H5) [NBP3-19654] - Western blot detection of HMGCL in Hela cell lysates using NBP3-19654 (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
S08-7H5
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

HMGCL Antibody (S08-7H5) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human HMGCL (Uniprot # P35914)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HMGCL
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1/1000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for HMGCL Antibody (S08-7H5)

  • 3-hydroxy-3-methylglutaryl-CoA lyase
  • 3-hydroxymethyl-3-methylglutaryl-CoA lyase
  • EC 4.1.3.4
  • HLmitochondrial

Background

The protein encoded by the HMGCL gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes thefinal step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associatedwith HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been foundfor this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HMGCL