![Western Blot: Histone H3.3 Antibody [NBP2-14981] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Western-Blot-NBP2-14981-img0010.jpg "Western Blot: Histone H3.3 Antibody [NBP2-14981] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
| Immunogen | Full length human Histone H3.3 Recombinant protein. |
| Marker | Marker of Transcriptionally Active Chromatin |
| Predicted Species | Porcine (100%), Bovine (100%), Rabbit (100%), Zebrafish (99%), Xenopus (100%), Monkey (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H3F3A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | ICC/IF-Assay dependent |
| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol (pH7). |
| Preservative | 0.025% Proclin 300 |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H3.3 Antibody (NBP2-14981)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | H3F3A |
![Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody [NBP2-14981] - Histone H3.3 antibody detects Histone H3.3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3.3 stained by Histone H3.3 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14981-img0009.jpg "Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody [NBP2-14981] - Histone H3.3 antibody detects Histone H3.3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3.3 stained by Histone H3.3 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-14981] - Histone H3.3 antibody detects Histone H3.3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Histone H3.3 stained by Histone H3.3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Immunohistochemistry-Paraffin-NBP2-14981-img0008.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-14981] - Histone H3.3 antibody detects Histone H3.3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Histone H3.3 stained by Histone H3.3 antibody diluted at 1:500.")
![Western Blot: Histone H3.3 Antibody [NBP2-14981] - A. 30 ug NIH-3T3 whole cell lysate/extractB. 30 ug JC whole cell lysate/extractC. 30 ug BCL-1 whole cell lysate/extractD. 30 ug C2C12 whole cell lysate/extractE. 30 ug Raw264.7 whole cell lysate/extract15 % SDS-PAGE Histone H3.3 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Western-Blot-NBP2-14981-img0004.jpg "Western Blot: Histone H3.3 Antibody [NBP2-14981] - A. 30 ug NIH-3T3 whole cell lysate/extractB. 30 ug JC whole cell lysate/extractC. 30 ug BCL-1 whole cell lysate/extractD. 30 ug C2C12 whole cell lysate/extractE. 30 ug Raw264.7 whole cell lysate/extract15 % SDS-PAGE Histone H3.3 antibody dilution: 1:10000")
![Western Blot: Histone H3.3 Antibody [NBP2-14981] - A. 30 ug PC-12 whole cell lysate/extract 15 % SDS-PAGEHistone H3.3 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Western-Blot-NBP2-14981-img0005.jpg "Western Blot: Histone H3.3 Antibody [NBP2-14981] - A. 30 ug PC-12 whole cell lysate/extract 15 % SDS-PAGEHistone H3.3 antibody dilution: 1:10000")
![Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-14981] - Sample: Paraffin-embedded mouse middle brain. Histone H3.3 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H3.3-Antibody-Immunohistochemistry-Paraffin-NBP2-14981-img0007.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-14981] - Sample: Paraffin-embedded mouse middle brain. Histone H3.3 antibody dilution: 1:500.")
![Western Blot HIST1H3A [ac Lys18] Antibody](https://images.novusbio.com/images/HIST1H3A-[ac-Lys18]-Antibody-Western-Blot-NBP2-43535-img0010.jpg)
![Immunohistochemistry-Paraffin HIST1H3A [ac Lys18] Antibody](https://images.novusbio.com/images/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420122076.jpg)
![Immunocytochemistry/ Immunofluorescence HIST1H3A [ac Lys18] Antibody](https://images.novusbio.com/images/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014689.jpg)
![Knockout Validated Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Knockout_Validated_22540.jpg)
![Immunocytochemistry Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Immunocytochemistry__Immunofluorescence_19067.jpg)
![Western Blot Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Western_Blot_13853.jpg)