Histone H3 [p Ser28] Antibody (HTA28)

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IF/IHC, IHC-Fr.

Showing Publications 1 - 10 of 10.

Publications using NB600-1168	Applications	Species
Jarrosson L, Costechareyre C, Gallix F Et al. An avian embryo patient-derived xenograft model for preclinical studies of human breast cancers iScience 2021-12-01 [PMID: 34849474] (IF/IHC, Human)	IF/IHC	Human
Koopmans T, van Beijnum H, Roovers EF et al. Ischemic tolerance and cardiac repair in the spiny mouse (Acomys) NPJ Regen Med 2021-11-17 [PMID: 34789755] Details: Citation using the Alexa Fluor 647 format of this antibody.
Hoshino A, Ratnapriya R, Brooks MJ et al. Molecular Anatomy of the Developing Human Retina. Dev. Cell 2017-12-18 [PMID: 29233477] (Human)		Human
Kicheva A, Bollenbach T, Ribeiro A et al. Coordination of progenitor specification and growth in mouse and chick spinal cord. Science. 2014-09-26 [PMID: 25258086] (IF/IHC)	IF/IHC
Stevens, HE et al. Fgfr2 Is Required For The Development Of The Medial Prefrontal Cortex And Its Connections With Limbic Circuits J. Neurosci 30, 5590 - 5602. 2010-01-01 [PMID: 20410112]
Georgi SA, Reh TA. Dicer is required for the transition from early to late progenitor state in the developing mouse retina. J Neurosci. 2010-03-17 [PMID: 20237275] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Yin Y, White AC, Huh SH et al. An FGF-WNT gene regulatory network controls lung mesenchyme development. Dev Biol. 2008-07-01 [PMID: 18533146]
Smith, A et al. FGF stimulation of the Erk1/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment. Development 134, 2895-2902. 2007-01-01 [PMID: 17660198]
Mateescu, B et al. Tethering of HP1 proteins to chromatin is relieved by phosphoacetylation of histone H3 EMBO Rep. 5, 490-496. 2004-01-01 [PMID: 15105826]
Goto, H. Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation. J Biol. [PMID: 10464286]

Array NB600-1168

• Histone H3 Antibodies
• Histone H3 Proteins