Histone H3 [Trimethyl Lys9] Antibody (6F12-H4)

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Invertebrate, Porcine, Yeast.

We have publications tested in 7 applications: Chemotaxis, ICC/IF, IF/IHC, IHC-P, IP, PLA, WB.

Showing Publications 1 - 10 of 27. Show All 27 Publications. Collapse Publications.

Publications using NBP1-30141	Applications	Species
Malla AB, Yu H, Farris D et al. DOT1L bridges transcription and heterochromatin formation at mammalian pericentromeres EMBO reports 2023-06-15 [PMID: 37317657]
Sun X, Eastman G, Shi Y et al. Structural and functional damage to neuronal nuclei caused by extracellular tau oligomers bioRxiv : the preprint server for biology 2023-05-08 [PMID: 37214909] (WB)	WB
Legartov� S, Lochmanov� G, B�rtov� E. The Highest Density of Phosphorylated Histone H1 Appeared in Prophase and Prometaphase in Parallel with Reduced H3K9me3, and HDAC1 Depletion Increased H1.2/H1.3 and H1.4 Serine 38 Phosphorylation Life (Basel) 2022-05-27 [PMID: 35743829] (ICC/IF)	ICC/IF
Marsoner T, Yedavalli P, Masnovo C et al. Aurora B activity is promoted by cooperation between discrete localization sites in budding yeast Molecular biology of the cell 2022-06-15 [PMID: 35704464]
Hollenstein DM, Veis J, Romanov N et al. PP2ARts1 antagonizes Rck2-mediated hyperosmotic stress signaling in yeast Microbiological research [PMID: 35461031] (WB, Yeast)	WB	Yeast
Saleh T, El-Sadoni M, Alhesa A Et al. Expression of Senescence and Apoptosis Biomarkers in Synchronous Bilateral Breast Cancer: A Case Report Current oncology (Toronto, Ont.) 2021-09-30 [PMID: 34677245] (IF/IHC, Human)	IF/IHC	Human
Hollenstein DM, GErecovA G, Romanov N Et al. A phosphatase-centric mechanism drives stress signaling response EMBO reports 2021-11-04 [PMID: 34558777] (PLA, Yeast)	PLA	Yeast
Bozic J, Motaln H, JaneZ AP et al. Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA Brain : a journal of neurology 2021-09-17 [PMID: 34534264] (WB, Human)	WB	Human
Saleh T, Alhesa A, Al-Balas M et al. Expression of therapy-induced senescence markers in breast cancer samples upon incomplete response to neoadjuvant chemotherapy Bioscience reports 2021-05-28 [PMID: 33948615] (IF/IHC, Human)	IF/IHC	Human
Perea-GarcIa A, MirO P, JimEnez-Lorenzo R et al. Sequential recruitment of the mRNA decay machinery to the iron-regulated protein Cth2 in Saccharomyces cerevisiae Biochim Biophys Acta Gene Regul Mech 2020-06-19 [PMID: 32565401] (WB, Yeast) Details: Saccharomyces cerevisia	WB	Yeast
Qian Y, Li Y, Zheng C et al. High Methylation Levels of Histone H3 lysine 9 Associated with Activation of HIF-1 alpha Predict Patients’ Worse Prognosis in Human Hepatocellular Carcinomas Cancer Genetics 2020-05-01 [PMID: 32534446] (IHC-P, Human)	IHC-P	Human
Hollenstein DM, GOmez-SAnchez R, Ciftci A Vac8 spatially confines autophagosome formation at the vacuole in S. cerevisiae J. Cell. Sci. 2019-11-14 [PMID: 31649143] (WB, Yeast)	WB	Yeast
van Drogen F, Mishra R, Rudolf F et al. Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 J. Cell Biol. 2019-07-17 [PMID: 31315942]
Janschitz M, Romanov N et al. Novel interconnections of HOG signaling revealed by combined use of two proteomic software packages. Cell Commun Signal 2019-06-17 [PMID: 31208443] (WB, Human)	WB	Human
Zhou M, Li Y, Lin S et al. H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers Dig. Dis. Sci. 2019-02-20 [PMID: 30788686] (IHC-P, Human)	IHC-P	Human
Zhao C, Shi J, Zhou R et al. DZNep and UNC0642 enhance in vitro developmental competence of cloned pig embryos Reproduction 2019-02-01 [PMID: 30730848] (ICC/IF, Porcine)	ICC/IF	Porcine
Stixova L, Komurkova D, Svobodova Kovarikova A, Bartova E. UVA irradiation strengthened an interaction between UBF1/2 proteins and H4K20 di-/tri-methylation. Chromosome Res. 2019-01-04 [PMID: 30610403] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Li Y, Guo D, Sun R et al. Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig. Dis. Sci. 2018-10-22 [PMID: 30350241] (IHC-P, Human)	IHC-P	Human
Peterson JM, Wang DJ, Shettigar V et al. NF-kB inhibition rescues cardiac function by remodeling calcium genes in a Duchenne muscular dystrophy model Nat Commun 2018-08-24 [PMID: 30143619] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Kai Y, Li Y, Sun T et al. A medial prefrontal cortex-nucleus acumens corticotropin-releasing factor circuitry for neuropathic pain-increased susceptibility to opioid reward Transl Psychiatry 2018-05-21 [PMID: 29780165] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Romanov N, Hollenstein DM, Janschitz M et al. Identifying Protein Kinase Specific Effectors of the Osmostress Response in Yeast Sci Signal. [PMID: 28270554] (IP, Yeast)	IP	Yeast
Nandakumar V, Hansen N, Glenn HL et al. Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep. 2016-08-09 [PMID: 27503568] (ICC/IF, Human)	ICC/IF	Human
No JG, Choi MK, Kwon DJ et al. Cell-free extract from porcine induced pluripotent stem cells can affect porcine somatic cell nuclear reprogramming. J Reprod Dev 2015-01-01 [PMID: 25736622] (WB, Porcine)	WB	Porcine
Krapivinsky G, Krapivinsky L, Manasian Y, Clapham DE. The TRPM7 Chanzyme Is Cleaved to Release a Chromatin-Modifying kinase. Cell. 2014-05-22 [PMID: 24855944] (WB, Mouse)	WB	Mouse
Herraiz A, Belles X, Piulachs MD et al. Chorion formation in panoistic ovaries requires windei and trimethylation of histone 3 lysine 9. Exp Cell Res 2013-07-18 [PMID: 23872316] (IF/IHC, ICC/IF, Invertebrate)	IF/IHC, ICC/IF	Invertebrate
Cornacchia D, Dileep V, Quivy JP et al. Mouse Rif1 is a key regulator of the replication-timing programme in mammalian cells EMBO J 2012-07-31 [PMID: 22850673] (WB, Mouse)	WB	Mouse
Tan J, Yang X, Zhuang L et al. Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells. Genes Dev. 2007-01-01 [PMID: 17437993]
Show All 27 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	ICC	Human	03/11/2022	View Summary Application Immunocytochemistry Sample Tested A549 cells Species Human Lot B-1
Enlarge	5 reviewed by: Verified Customer	WB	Human	09/09/2014	View Summary Application Western Blot Sample Tested H3K9(me)3 immunoblot in MCF7 and HCT116 Species Human

Array NBP1-30141

• Histone H3 Antibodies
• Histone H3 Proteins