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HepaCAM Antibodies

We offer HepaCAM Antibodies for use in common research applications: Western Blot. Each HepaCAM Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HepaCAM Antibodies can be used in a variety of model species: Human. Use the list below to choose the HepaCAM Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HepaCAM polyclonal antibodies and browse our HepaCAM monoclonal antibody catalog.

Alternate Names for HepaCAM Antibodies

anti-HepaCAM antibody, anti-cancer susceptibility antibody, anti-FLJ25530 antibody, anti-glial cell adhesion molecule antibody, anti-GlialCAM antibody, anti-hepatic and glial cell adhesion molecule antibody, anti-hepatocyte cell adhesion molecule antibody, anti-Protein hepaCAM antibody

5 Results for "hepacam" in Primary Antibodies

MAB4108

HepaCAM Antibody (419305) [Unconjugated]

(1 Review)

(15 Publications)

100 μg

MAB4108

Western blot shows lysates of human brain (motor cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human HepaCAM Monoclonal Antibody (Catalog # MAB4108) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HepaCAM at approximately 45-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HepaCAM associates with connexin 43.(A) U373 MG cells were stably transfected with pcDNA3.1 vector, wild-type hepaCAM, hepaCAM-R92Q and hepaCAM-R92W. Immunofluorescent staining was performed with antibodies against the hepaCAM cytoplasmic domain (green) and connexin 43 (red). Co-localization of hepaCAM and connexin 43 is indicated by yellow fluorescence. Nuclei were stained with DAPI (blue). Insets show a higher magnification of sites of cell-cell contacts. Cells were visualized by confocal microscopy under a 60× objective. Scale bar: 10 μm. (B) Co-immunoprecipitatation of connexin 43 and hepaCAM. Cell lysates were prepared from U373 MG cells stably transfected with pcDNA3.1 vector and wild-type hepaCAM, and immunoprecipitated with antibody against the hepaCAM extracellular domain (IP hepaCAM). Immunoprecipitation with mouse IgG1 (IP IgG) was included as a negative control. Western blot analysis was performed on the immunoprecipitates and input (3%) using connexin 43 antibody. The efficiency of hepaCAM immunoprecipitation was evaluated with an HRP-conjugated FLAG antibody. The IgG heavy chain detected with an HRP-conjugated anti-mouse antibody is shown as a loading control. (C) Co-immunoprecipitation of wild-type and mutant hepaCAM with connexin 43. Cell lysates were immunoprecipitated with antibody against the hepaCAM extracellular domain (IP hepaCAM). Immunoprecipitation with mouse IgG1 (IP IgG) was included as a negative control. Western blot analysis was performed on the immunoprecipitates and input (2%) using connexin 43 antibody. (D) Expression of wild-type hepaCAM increases connexin 43 protein levels in U373 MG cells. 20 μg of cell lysates were subjected to Western blot analysis. GAPDH was used as a loading control. The result presented is a representative experiment of four independent experiments with similar results. The full view blots are shown in Supplementary Figure 1. (E) Quantification of connexin 43 protein levels in D and in three additional independent Western blot analyses. Using ImageJ the densities of the connexin 43 bands were normalized to the densities of the respective GAPDH bands for each sample, and the mean relative density over the four experiments was calculated. The data presented are the means ± SE (n = 4), **p < 0.01 as assessed by one-way ANOVA with Tukey’s multiple comparison test. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36218), licensed under a CC-BY license. Not internally tested by R&D Systems.

HepaCAM regulates connexin 43 stability.(A) Evaluation of connexin 43 mRNA expression. Total RNA was analyzed by RT-PCR. GAPDH and no template controls (NTC) were included as housekeeping gene and negative controls, respectively. (B) Evaluation of connexin 43 protein stability by a cycloheximide (CHX) chase assay. Cells treated with CHX (50 μg/ml) for the times indicated were lysed and 30 μg of cell lysates were subjected to Western blot analysis. The result presented is a representative experiment of three independent experiments with similar results. (C) Quantification of all three CHX chase experiments using ImageJ. The densities of the connexin 43 bands were normalized to the densities of the respective GAPDH bands at each time-point. The level of connexin 43 remaining at each time-point was calculated as a percentage of the initial connexin 43 level (time 0 of CHX treatment). The data presented are the means ± SE (n = 3). (D) Expression of hepaCAM in HEK293T cells increases connexin 43 protein levels. HEK293T cells were transiently transfected with pcDNA3.1 vector or wild-type hepaCAM. Two days after transfection, cells were lysed and 60 μg of cell lysates were subjected to Western blot analysis using antibodies against connexin 43 and the hepaCAM extracellular domain. The result presented is a representative experiment of three independent experiments with similar results. (E) Quantification of all three experiments using ImageJ. The densities of the connexin 43 bands were normalized to the densities of the respective GAPDH bands for each sample, and the mean relative density over the three experiments was calculated. The data presented are the means ± SE (n = 3), ***p < 0.0001 as assessed by t-test. (F) HepaCAM slows down connexin 43 turnover by the lysosomal pathway. U373 MG cells stably transfected with pcDNA3.1 vector or wild-type hepaCAM were treated with chloroquine (50 μM) and 30 μg of cell lysates were subjected to Western blot analysis for connexin 43. The result presented is representative of two independent experiments with similar results. The full view blots for (B,D,F) are shown in Supplementary Figures 3,4 and 5, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36218), licensed under a CC-BY license. Not internally tested by R&D Systems.

HepaCAM regulates connexin 43 stability.(A) Evaluation of connexin 43 mRNA expression. Total RNA was analyzed by RT-PCR. GAPDH and no template controls (NTC) were included as housekeeping gene and negative controls, respectively. (B) Evaluation of connexin 43 protein stability by a cycloheximide (CHX) chase assay. Cells treated with CHX (50 μg/ml) for the times indicated were lysed and 30 μg of cell lysates were subjected to Western blot analysis. The result presented is a representative experiment of three independent experiments with similar results. (C) Quantification of all three CHX chase experiments using ImageJ. The densities of the connexin 43 bands were normalized to the densities of the respective GAPDH bands at each time-point. The level of connexin 43 remaining at each time-point was calculated as a percentage of the initial connexin 43 level (time 0 of CHX treatment). The data presented are the means ± SE (n = 3). (D) Expression of hepaCAM in HEK293T cells increases connexin 43 protein levels. HEK293T cells were transiently transfected with pcDNA3.1 vector or wild-type hepaCAM. Two days after transfection, cells were lysed and 60 μg of cell lysates were subjected to Western blot analysis using antibodies against connexin 43 and the hepaCAM extracellular domain. The result presented is a representative experiment of three independent experiments with similar results. (E) Quantification of all three experiments using ImageJ. The densities of the connexin 43 bands were normalized to the densities of the respective GAPDH bands for each sample, and the mean relative density over the three experiments was calculated. The data presented are the means ± SE (n = 3), ***p < 0.0001 as assessed by t-test. (F) HepaCAM slows down connexin 43 turnover by the lysosomal pathway. U373 MG cells stably transfected with pcDNA3.1 vector or wild-type hepaCAM were treated with chloroquine (50 μM) and 30 μg of cell lysates were subjected to Western blot analysis for connexin 43. The result presented is representative of two independent experiments with similar results. The full view blots for (B,D,F) are shown in Supplementary Figures 3,4 and 5, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36218), licensed under a CC-BY license. Not internally tested by R&D Systems.

HepaCAM enhances connexin 43 expression.(A) Treatment of hepaCAM-expressing U373 MG cells with antibodies against the hepaCAM extracellular domain prevents the association of hepaCAM with connexin 43 at cell-cell contacts. Wild-type hepaCAM-expressing U373 MG cells were treated overnight with antibody against the hepaCAM extracellular domain in soluble form (10 μg/ml). Cells were also treated with the isotype mouse IgG1 as a control. The next day, cells were fixed and immunofluorescent staining was performed with antibodies against the hepaCAM extracellular domain (green) and connexin 43 (red). Co-localization of hepaCAM and connexin 43 is indicated by yellow fluorescence. Nuclei were stained with DAPI (blue). Cells were visualized by confocal microscopy under a 60× objective. Scale bar: 10 μm. (B) Treatment of hepaCAM-expressing U373 MG cells with antibodies against the hepaCAM extracellular domain causes a downregulation of connexin 43 expression. Wild-type hepaCAM-expressing U373 MG cells were treated overnight with antibody against the hepaCAM extracellular domain in soluble form (10 μg/ml). Cells were also treated with the isotype mouse IgG1 as a control. The next day, cells were lysed and 20 μg of cell lysates were subjected to Western blot analysis using connexin 43 antibody. GAPDH was used as a loading control. The full view blots are shown in Supplementary Figure 2. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36218), licensed under a CC-BY license. Not internally tested by R&D Systems.

HepaCAM associates with connexin 43.(A) U373 MG cells were stably transfected with pcDNA3.1 vector, wild-type hepaCAM, hepaCAM-R92Q and hepaCAM-R92W. Immunofluorescent staining was performed with antibodies against the hepaCAM cytoplasmic domain (green) and connexin 43 (red). Co-localization of hepaCAM and connexin 43 is indicated by yellow fluorescence. Nuclei were stained with DAPI (blue). Insets show a higher magnification of sites of cell-cell contacts. Cells were visualized by confocal microscopy under a 60× objective. Scale bar: 10 μm. (B) Co-immunoprecipitatation of connexin 43 and hepaCAM. Cell lysates were prepared from U373 MG cells stably transfected with pcDNA3.1 vector and wild-type hepaCAM, and immunoprecipitated with antibody against the hepaCAM extracellular domain (IP hepaCAM). Immunoprecipitation with mouse IgG1 (IP IgG) was included as a negative control. Western blot analysis was performed on the immunoprecipitates and input (3%) using connexin 43 antibody. The efficiency of hepaCAM immunoprecipitation was evaluated with an HRP-conjugated FLAG antibody. The IgG heavy chain detected with an HRP-conjugated anti-mouse antibody is shown as a loading control. (C) Co-immunoprecipitation of wild-type and mutant hepaCAM with connexin 43. Cell lysates were immunoprecipitated with antibody against the hepaCAM extracellular domain (IP hepaCAM). Immunoprecipitation with mouse IgG1 (IP IgG) was included as a negative control. Western blot analysis was performed on the immunoprecipitates and input (2%) using connexin 43 antibody. (D) Expression of wild-type hepaCAM increases connexin 43 protein levels in U373 MG cells. 20 μg of cell lysates were subjected to Western blot analysis. GAPDH was used as a loading control. The result presented is a representative experiment of four independent experiments with similar results. The full view blots are shown in Supplementary Figure 1. (E) Quantification of connexin 43 protein levels in D and in three additional independent Western blot analyses. Using ImageJ the densities of the connexin 43 bands were normalized to the densities of the respective GAPDH bands for each sample, and the mean relative density over the four experiments was calculated. The data presented are the means ± SE (n = 4), **p < 0.01 as assessed by one-way ANOVA with Tukey’s multiple comparison test. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36218), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4108-SP

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NBP3-04983

HepaCAM Antibody - Azide and BSA Free

(1 Publication)

100 ul

NBP3-04983

Western Blot: HepaCAM Antibody [NBP3-04983] - Analysis of extracts of various cell lines, using HepaCAM antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-04983-20ul

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NBP1-60020

HepaCAM Antibody

100 ul

NBP1-60020

Western Blot: HEPACAM Antibody [NBP1-60020] - HT1080 cell lysate, concentration 0.2-1 ug/ml.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-09339

HepaCAM Antibody

100 ul

NBP3-09339

Western Blot: HepaCAM Antibody [NBP3-09339] - Western blot analysis of HepaCAM in Rat Spleen lysates. Antibody dilution at 1.0ug/ml

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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BAF4108

HepaCAM Antibody [Biotin]

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Conjugate:

Biotin

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4108
Carrier Free	AF4108-SP

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