![Western Blot: HDAC3 Antibody [NBP2-16791] - HDAC3 antibody detects HDAC3 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0015.jpg "Western Blot: HDAC3 Antibody [NBP2-16791] - HDAC3 antibody detects HDAC3 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted by 1:1000.")
| Reactivity | Hu, Mu, Rt, Ze, Po, Bv, Ch, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HDAC3. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (95%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HDAC3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HDAC3 Antibody (NBP2-16791)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NBP2-16791] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16791-img0012.jpg "Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NBP2-16791] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP2-16791] - Immunohistochemical analysis of paraffin-embedded OVCA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunohistochemistry-Paraffin-NBP2-16791-img0002.jpg "Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP2-16791] - Immunohistochemical analysis of paraffin-embedded OVCA, using antibody at 1:250 dilution.")
![Chromatin Immunoprecipitation: HDAC3 Antibody [NBP2-16791] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-HDAC3 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Chromatin-Immunoprecipitation-NBP2-16791-img0011.jpg "Chromatin Immunoprecipitation: HDAC3 Antibody [NBP2-16791] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-HDAC3 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.")
![Western Blot: HDAC3 Antibody [NBP2-16791] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0004.jpg "Western Blot: HDAC3 Antibody [NBP2-16791] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0007.jpg "Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0009.jpg "Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
![Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0013.jpg "Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0014.jpg "Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunoprecipitation: HDAC3 Antibody [NBP2-16791] - 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 ug of HDAC3 antibody 7.5% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunoprecipitation-NBP2-16791-img0005.jpg "Immunoprecipitation: HDAC3 Antibody [NBP2-16791] - 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 ug of HDAC3 antibody 7.5% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")