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Haptoglobin Antibody (OTI4H5) - Azide and BSA Free

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Western Blot: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Haptoglobin (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Haptoglobin.
Flow Cytometry: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by ...read more
Western Blot: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Haptoglobin monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, CyTOF-ready
Clone
OTI4H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

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Haptoglobin Antibody (OTI4H5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human HP (NP_005134) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500
Theoretical MW
43.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Haptoglobin Antibody (OTI4H5) - Azide and BSA Free

  • binding peptide
  • BP
  • haptoglobin alpha(1S)-beta
  • haptoglobin alpha(2FS)-beta
  • Haptoglobin
  • haptoglobin, alpha polypeptide
  • haptoglobin, beta polypeptide
  • HP
  • HP-1
  • hp2-alpha
  • HP2ALPHA2
  • HPA1S
  • MGC111141
  • zonulin

Background

Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HP