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HAND1 Antibody [Unconjugated]

 (13 Publications)
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Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HAND1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody ...read more
HAND1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) at 5 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3168
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3168-SP
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HAND1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HAND1
Met1-Gln215
Accession # O96004
Specificity
Detects human HAND1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HAND2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HAND1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3168 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAND1 Antibody [Unconjugated]

  • BHLHA27
  • bHLHa27heart- and neural crest derivatives-expressed protein 1
  • Class A basic helix-loop-helix protein 27
  • eHand
  • Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1
  • HAND1
  • heart and neural crest derivatives expressed 1
  • Hxt
  • Thing1

Background

HAND1 belongs to the HAND family of conserved basic helix-loop-helix transcription factors. It is expressed in the heart, ganglia, neural crest derivatives and extraembryonic tissues. HAND1 plays an essential role in heart development and is critical for the specification of extraembryonic tissues including trophoblasts. Human and mouse HAND1 share approximately 92% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HAND1 Antibody (AF3168)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.


Filter By Application
Flow Cytometry
(3)
ICC
(8)
Western Blot
(1)
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Human
(8)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF3168 Applications Species
Stanberry, I;Cunningham, D;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B; Characterization of an induced pluripotent stem cell line NCHi011-A from a 23-year-old female with Alagille Syndrome harboring a heterozygous JAG1 pathogenic variant Stem cell research 2023-09-23 [PMID: 37774637] (ICC, Human) ICC Human
Cunningham, D;Stanberry, I;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B; Generation of iPSC line NCHi012-A from a patient with Alagille syndrome and heterozygous pathogenic variant in the JAG1 gene Stem cell research 2023-08-01 [PMID: 37549562] (ICC, Human) ICC Human
Marina Gattiglio, Michelle Protzek, Christian Schröter Population-level antagonism between FGF and BMP signaling steers mesoderm differentiation in embryonic stem cells Biology Open 2023-08-15 [PMID: 37530863]
Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm et al. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay Cell Reports Methods 2023-06-26 [PMID: 37426764]
Aracely Castillo-Venzor, Christopher A Penfold, Michael D Morgan, Walfred WC Tang, Toshihiro Kobayashi, Frederick CK Wong et al. Origin and segregation of the human germline Life Science Alliance 2023-08-01 [PMID: 37217306]
S Teague, G Primavera, B Chen, E Freeburne, H Khan, K Jo, C Johnson, I Heemskerk The time integral of BMP signaling determines fate in a stem cell model for early human development bioRxiv : the preprint server for biology, 2023-04-10;0(0):. 2023-04-10 [PMID: 37090515] (ICC, Human) ICC Human
EY Akda?, S Turan, D Guhathakur, A Ekici, S Salar, DC Lie, B Winner, A Fejtova CRISPR/Cas9-mediated generation of hESC lines with homozygote and heterozygote p.R331W mutation in CTBP1 to model HADDTS syndrome Stem Cell Research, 2022-12-30;67(0):103012. 2022-12-30 [PMID: 36610307] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD) Stem Cell Research, 2022-10-31;65(0):102958. 2022-10-31 [PMID: 36343514] (ICC, Human) ICC Human
M Ohgushi, N Taniyama, A Vandenbon, M Eiraku Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model Cell Reports, 2022-06-21;39(12):110973. 2022-06-21 [PMID: 35732132] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
Kazutoshi Takahashi, Chikako Okubo, Michiko Nakamura, Mio Iwasaki, Yuka Kawahara, Tsuyoshi Tabata et al. A stress-reduced passaging technique improves the viability of human pluripotent cells Cell Reports Methods 2022-02-28 [PMID: 35474962]
Show All 13 Publications.

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Bioinformatics

Gene Symbol HAND1
Uniprot