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HAND1 Antibody [Biotin]

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HAND1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human HAND1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3168) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
HAND1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human HAND1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3168) at 15 µg/mL for 3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF3168
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF3168
Carrier Free
AF3168
Carrier Free
AF3168-SP
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HAND1 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human HAND1
Met1-Gln215
Accession # O96004
Specificity
Detects human human HAND1 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human HAND2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HAND1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAND1 Antibody [Biotin]

  • BHLHA27
  • bHLHa27heart- and neural crest derivatives-expressed protein 1
  • Class A basic helix-loop-helix protein 27
  • eHand
  • Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1
  • HAND1
  • heart and neural crest derivatives expressed 1
  • Hxt
  • Thing1

Background

HAND1 belongs to the HAND family of conserved basic helix-loop-helix transcription factors. It is expressed in the heart, ganglia, neural crest derivatives and extraembryonic tissues. HAND1 plays an essential role in heart development and is critical for the specification of extraembryonic tissues including trophoblasts. Human and mouse HAND1 share approximately 92% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HAND1
Uniprot