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HABP1/C1QBP/GC1q R Antibody [Unconjugated]

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Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Nalm-6 human Pre-B acute lymphocytic leukemia cell line, human liver tissue, mouse brain tissue and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat HABP1/C1QBP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5359) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HABP1/C1QBP at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Nalm-6 human Pre-B acute lymphocytic leukemia cell line, human liver tissue, mouse brain tissue and rat testis tissue. PVDF membrane was probed ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for HABP1/C1QBP at approximately 34 kDa (as ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5359-SP
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HABP1/C1QBP/GC1q R Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse HABP1/C1QBP 
Leu72-Gln279
Accession # NP_031599
Specificity
Detects human, mouse, and rat HABP1/C1QBP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human C1QR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
C1QBP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF5359 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HABP1/C1QBP/GC1q R Antibody [Unconjugated]

  • C1q globular domain-binding protein
  • C1QBP
  • complement component 1 Q subcomponent-binding protein, mitochondrial
  • complement component 1, q subcomponent binding protein
  • gC1qBP
  • GC1q-R protein
  • gC1qR
  • gC1Q-R
  • Glycoprotein gC1qBP
  • HABP1
  • HABP1p33
  • Hyaluronan-binding protein 1
  • Mitochondrial matrix protein p32
  • p32
  • SF2p32
  • splicing factor SF2-associated protein

Background

HABP1 (hyaluronan binding protein1; also p32 and gC1qR) is a 33 kDa member of the MAM33 family of proteins. It is widely expressed and found on/in endothelial cells, platelets and dendritic cells. It has multiple binding partners, including complement C1q, hyaluronan, vitronectin and ARF. Full-length mouse HABP1 proprecursor is 279 amino acid (aa) in length. It contains a 70 aa mitochondrial targeting sequence (aa 1‑70) that is cleaved to generate a 209 aa mature segment. A hyaluronan binding site lies between Lys116‑Lys125 and a Tyr phosphorylation site exists at Tyr184. Although the prosegment is usually cleaved, the intact proprecursor is associated with sperm. HABP1 reportedly forms noncovalent homotrimers. Over aa 72‑279, mouse HABP1 shares 99% and 91% aa identity with rat and human HABP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HABP1/C1QBP/GC1q R Antibody (AF5359)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol C1QBP
Uniprot