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GSK-3 beta Antibody (272536) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
272536
Clonality
Monoclonal
Host
Rat
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2506U-100UG
Alexa Fluor 405
IC2506V-100UG
Alexa Fluor 488
IC2506G-100UG
Alexa Fluor 532
FAB2506X
Alexa Fluor 594
IC2506T-100UG
Alexa Fluor 647
IC2506R-100UG
Alexa Fluor 700
IC2506N-100UG
Alexa Fluor 750
IC2506S-100UG
Allophycocyanin
FAB2506A
Biotin
FAB2506B
DyLight 350
FAB2506D
DyLight 405
FAB2506E
DyLight 488
FAB2506K
DyLight 550
FAB2506L
DyLight 594
FAB2506M
DyLight 650
FAB2506W
DyLight 680
FAB2506Y
DyLight 755
FAB2506Z
Fluorescein
IC2506F
HRP
FAB2506H
Janelia Fluor 525
FAB2506JF525
Janelia Fluor 549
FAB2506I
Janelia Fluor 585
FAB2506JF585
Janelia Fluor 635
FAB2506JF635
Janelia Fluor 646
FAB2506J
Janelia Fluor 669
FAB2506JF669
PE
FAB2506P
PerCP
FAB2506C
Unconjugated
MAB2506
mFluor Violet 450 SE
FAB2506MFV450
mFluor Violet 500 SE
FAB2506MFV500
mFluor Violet 610 SE
FAB2506MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2506
Carrier Free
MAB2506
Carrier Free
IC2506F
Carrier Free
MAB2506-SP
Carrier Free
IC2506V-100UG
Carrier Free
IC2506U-100UG
Carrier Free
IC2506T-100UG
Carrier Free
IC2506S-100UG
Carrier Free
IC2506R-100UG
Carrier Free
IC2506N-100UG
Carrier Free
IC2506G-100UG
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GSK-3 beta Antibody (272536) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
E. coli-derived recombinant human GSK-3 beta
Met1-Thr420
Accession # P49841
Specificity
Detects human, mouse, and rat GSK-3 beta  in ELISAs and Western blots. Does not cross-react with recombinant human GSK-3 alpha .
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
GSK3B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry
  • Intracellular Staining by Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for GSK-3 beta Antibody (272536) [CoraFluor™ 1]

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen synthase kinase 3beta (GSK-3 beta) is a Serine/Threonine protein kinas involved in the insulin and wingless pathways (1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK-3 beta activity is down regulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Allzheimer's disease. Additionally, increased GSK-3 beta protein levels are found in Alzheimer's disease brains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GSK3B