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Human Growth Hormone R ELISA Kit (Colorimetric)

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ELISA: Human Growth Hormone R ELISA Kit (Colorimetric) [NBP2-68007] - Samples were spiked with high concentrations of Human Growth Hormone R and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Growth Hormone R ELISA Kit (Colorimetric) [NBP2-68007] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL

Order Details

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Human Growth Hormone R ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human GHR in samples. No significant cross-reactivity or interference between Human GHR and analogues was observed.
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.71%
Intra-Assay
CV% < 5.47%
Spike Recovery
85-111%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GHR

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100×)
  3. Concentrated HRP Conjugate (100×)
  4. Concentrated Wash Buffer (25×)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate(Dismountable)
  7. Plate Sealer
  8. Product Description
  9. Reference Standard & Sample Diluent
  10. Reference Standard
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Growth Hormone R ELISA Kit (Colorimetric)

  • GH receptor
  • GHBP
  • GHR
  • growth hormone binding protein
  • Growth Hormone R
  • growth hormone receptor
  • serum binding protein
  • Somatotropin receptor

Background

Growth hormone receptor encodes a protein that is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. A common alternate allele of this gene, called GHRd3, lacks exon three and has been well-characterized. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. Other splice variants, including one encoding a soluble form of the protein (GHRtr), have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GHR