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Granzyme B Antibody (351927) [PerCP (Peridinin-chlorophyll Protein Complex)]

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NK-92 human natural killer lymphoma cell line was stained with Mouse Anti-Human Granzyme B PerCP-conjugated Monoclonal Antibody (Catalog # IC2906C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
NK-92 human natural killer lymphoma cell line was stained with Mouse Anti-Human Granzyme B PerCP-conjugated Monoclonal Antibody (Catalog # IC2906C, filled histogram) or isotype control antibody (Catalog # IC003C, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
351927
Clonality
Monoclonal
Host
Mouse
Conjugate
PerCP (Peridinin-chlorophyll Protein Complex)

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2906U-100UG
Alexa Fluor 405
IC2906V-100UG
Alexa Fluor 488
IC2906G
Alexa Fluor 532
FAB2906X
Alexa Fluor 594
IC2906T-100UG
Alexa Fluor 647
IC2906R-100UG
Alexa Fluor 700
IC2906N-100UG
Alexa Fluor 750
IC2906S-100UG
Allophycocyanin
FAB2906A
Biotin
FAB2906B
CoraFluor 1
FAB2906CL1
DyLight 350
FAB2906D
DyLight 405
FAB2906E
DyLight 488
FAB2906K
DyLight 550
FAB2906L
DyLight 594
FAB2906M
DyLight 650
FAB2906W
DyLight 680
FAB2906Y
DyLight 755
FAB2906Z
FITC
FAB2906F
HRP
FAB2906H
Janelia Fluor 525
FAB2906JF525
Janelia Fluor 549
FAB2906I
Janelia Fluor 646
FAB2906J
Janelia Fluor 669
FAB2906JF669
Phycoerythrin
IC2906P
Unconjugated
MAB2906
mFluor Violet 450 SE
FAB2906MFV450
mFluor Violet 500 SE
FAB2906MFV500
mFluor Violet 610 SE
FAB2906MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2906
Carrier Free
MAB2906
Carrier Free
IC2906G
Carrier Free
IC2906P
Carrier Free
MAB2906-SP
Carrier Free
IC2906V-100UG
Carrier Free
IC2906U-100UG
Carrier Free
IC2906T-100UG
Carrier Free
IC2906S-100UG
Carrier Free
IC2906R-100UG
Carrier Free
IC2906N-100UG
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Granzyme B Antibody (351927) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Granzyme B
Gly19-Tyr247
Accession # P10144
Specificity
Detects human Granzyme B in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Granzyme A, rhGranzyme H, recombinant mouse (rm) Granzyme B, rmGranzyme C, rmGranzyme D, or rmGranzyme G.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GZMB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publication using
IC2906C in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Granzyme B Antibody (351927) [PerCP (Peridinin-chlorophyll Protein Complex)]

  • C11
  • CCPI
  • CGL1
  • CGL-1
  • CSPB
  • CSP-B
  • CTLA1
  • CTLA-1
  • CTSGL1
  • Fragmentin-2
  • Granzyme B
  • Granzyme-2
  • GranzymeB
  • GRB
  • GrzB
  • GZMB
  • HLP
  • SECT

Background

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro peptide (residues 19-20), and a mature chain (residues 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of residues 19-247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).

  1. Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al., eds., pp. 1549 - 1552.
  4. Schmid, J. and C. Weissman (1987) J. Immunol. 139:250.
  5. Caputo, A. et al. (1988) J. Biol. Chem. 263:6363.
  6. Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Granzyme B
Antibody (351927) [Pe ...
IC2906C
Granzyme B Antibody (351927) [PerCP (Peridinin-chlorophyll Protein Complex)]
Species: Hu
Applications: Flow

Publications for Granzyme B Antibody (IC2906C)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol GZMB
Uniprot