Immunohistochemistry: GPR55 Antibody [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (b-f) immunolabeling. (a-c) Arrows indicate the Neurotrace-labeled nuclei ...read more
Western Blot: GPR55 Antibody [NB110-55498] - Representative image of Western blots (WB) analysis showing the specificity of the primary antibody rabbit anti-G protein-coupled receptor 55 (GPR55). The antibody revealed a ...read more
Immunohistochemistry-Paraffin: GPR55 Antibody [NB110-55498] - Analysis of anti-GPR55 antibody with human pancreas, islet of langerhans.
Immunohistochemistry-Frozen: GPR55 Antibody [NB110-55498] - Cryosection of canine cervical dorsal root ganglion in which satellite glial cells and some sensory neurons expressed GPR55 immunoreactivity. Image submitted ...read more
Immunocytochemistry/ Immunofluorescence: GPR55 Antibody - BSA Free [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (a–f) & PPARalpha (g–i) immunolabeling. ...read more
Immunocytochemistry/ Immunofluorescence: GPR55 Antibody - BSA Free [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (a–f) & PPARalpha (g–i) immunolabeling. ...read more
Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (95%), Marmoset (95%), Bovine (85%), Mouse (80%), Equine (80%), Porcine (80%). Canine reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for GPR55 Antibody - BSA Free
G protein-coupled receptor 55
Gm218
GPR55
G-protein coupled receptor 55
Background
G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR55 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, where mRNA transcripts were detected in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain. ESTs have been isolated from liver/spleen libraries.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
We have publications tested in 3 confirmed species: Canine, Equine, Horse.
We have publications tested in 8 applications: ICC/IF, IHC, IHC-P, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, WB, Western Blot.
FAQs for GPR55 Antibody (NB110-55498). (Showing 1 - 2 of 2 FAQs).
Do you have any data about the use of the following antibody in Immunofluorescence and Confocal Microscopy:
We have only validated product NB110-55498 for use in IHC Paraffin. Please see our images on the datasheet. The recommended dilution for this antibody in IHC-P is 5 ug/ml.
I'm interested in one of your products: GPR55 Antibody (NB110-55498). I'd like to know if it has ever been used in non permeabilised cells, indicating that it can efficiently recognize an extracellular epitope.
We have done validation of this product in IHC-P of pancreas as well as spleen tissues after heat-induced antigen retrieval and yes, permeabilization was executed before blocking/primary antibody incubation step in the protocol. Have you seen our another GPR55 antibody (NLS6817)? This antibody has been generated employing an immunogen which was a synthetic peptide made to the 3rd extracellular domain of GPR55 protein. Because this antibody should technically be detecting extra-cellular domain of the target, you may try skipping permeabilization step for this antibody, however, you will still need to perform antigen retrieval.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry: GPR55 Antibody [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (b-f) immunolabeling. (a-c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells which showed bright GPR55 immunolabelling (c). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d-f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) and glial fibrillary acidic protein (GFAP) immunoreactivity. Stars indicate sensory neurons of different dimension, which expressed faint-to-moderate GPR55 immunoreactivity. Open arrow indicate autofluorescent pigment. Bar: 50 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2019.00313/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPR55-Antibody-Immunohistochemistry-NB110-55498-img0005.jpg "Immunohistochemistry: GPR55 Antibody [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (b-f) immunolabeling. (a-c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells which showed bright GPR55 immunolabelling (c). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d-f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) and glial fibrillary acidic protein (GFAP) immunoreactivity. Stars indicate sensory neurons of different dimension, which expressed faint-to-moderate GPR55 immunoreactivity. Open arrow indicate autofluorescent pigment. Bar: 50 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2019.00313/full) licensed under a CC-BY license.")
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![Western Blot: GPR55 Antibody [NB110-55498] - Representative image of Western blots (WB) analysis showing the specificity of the primary antibody rabbit anti-G protein-coupled receptor 55 (GPR55). The antibody revealed a single band of expected molecular weight (~40 kDa). The images of the different immunoblots were slightly adjusted in brightness and contrast to match their backgrounds. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2019.00313/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPR55-Antibody-Western-Blot-NB110-55498-img0004.jpg "Western Blot: GPR55 Antibody [NB110-55498] - Representative image of Western blots (WB) analysis showing the specificity of the primary antibody rabbit anti-G protein-coupled receptor 55 (GPR55). The antibody revealed a single band of expected molecular weight (~40 kDa). The images of the different immunoblots were slightly adjusted in brightness and contrast to match their backgrounds. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2019.00313/full) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: GPR55 Antibody [NB110-55498] - Analysis of anti-GPR55 antibody with human pancreas, islet of langerhans.](http://images.novusbio.com/fullsize/GPR55-Antibody-Immunohistochemistry-Paraffin-NB110-55498-img0002.jpg "Immunohistochemistry-Paraffin: GPR55 Antibody [NB110-55498] - Analysis of anti-GPR55 antibody with human pancreas, islet of langerhans.")
![Immunohistochemistry-Frozen: GPR55 Antibody [NB110-55498] - Cryosection of canine cervical dorsal root ganglion in which satellite glial cells and some sensory neurons expressed GPR55 immunoreactivity. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GPR55-Antibody-Immunohistochemistry-Frozen-NB110-55498-img0003.jpg "Immunohistochemistry-Frozen: GPR55 Antibody [NB110-55498] - Cryosection of canine cervical dorsal root ganglion in which satellite glial cells and some sensory neurons expressed GPR55 immunoreactivity. Image submitted by a verified customer review.")
![Immunocytochemistry/ Immunofluorescence: GPR55 Antibody - BSA Free [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (a–f) & PPARalpha (g–i) immunolabeling. (a–c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells (a) which showed bright GPR55 immunolabelling (b). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d–f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) & glial fibrillary acidic protein (GFAP) immunoreactivity; open arrows indicate SGCs which were GPR55 immunoreactive & GFAP negative (e). Stars indicate sensory neurons of different dimension, which expressed faint –to-moderate GPR55 immunoreactivity. (g–i) White arrows indicate the Neurotrace labeled nuclei of SGCs which showed PPARalpha immunoreactivity (h). Open arrow indicate autofluorescent pigment. Bar: a–i = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31608295), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-55498_rabbit-gpr55-pab-bsa-free-310202416235511.jpg "Immunocytochemistry/ Immunofluorescence: GPR55 Antibody - BSA Free [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (a–f) & PPARalpha (g–i) immunolabeling. (a–c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells (a) which showed bright GPR55 immunolabelling (b). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d–f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) & glial fibrillary acidic protein (GFAP) immunoreactivity; open arrows indicate SGCs which were GPR55 immunoreactive & GFAP negative (e). Stars indicate sensory neurons of different dimension, which expressed faint –to-moderate GPR55 immunoreactivity. (g–i) White arrows indicate the Neurotrace labeled nuclei of SGCs which showed PPARalpha immunoreactivity (h). Open arrow indicate autofluorescent pigment. Bar: a–i = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31608295), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: GPR55 Antibody - BSA Free [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (a–f) & PPARalpha (g–i) immunolabeling. (a–c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells (a) which showed bright GPR55 immunolabelling (b). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d–f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) & glial fibrillary acidic protein (GFAP) immunoreactivity; open arrows indicate SGCs which were GPR55 immunoreactive & GFAP negative (e). Stars indicate sensory neurons of different dimension, which expressed faint –to-moderate GPR55 immunoreactivity. (g–i) White arrows indicate the Neurotrace labeled nuclei of SGCs which showed PPARalpha immunoreactivity (h). Open arrow indicate autofluorescent pigment. Bar: a–i = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31608295), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-55498_rabbit-gpr55-pab-bsa-free-31020241623557.jpg "Immunocytochemistry/ Immunofluorescence: GPR55 Antibody - BSA Free [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (a–f) & PPARalpha (g–i) immunolabeling. (a–c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells (a) which showed bright GPR55 immunolabelling (b). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d–f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) & glial fibrillary acidic protein (GFAP) immunoreactivity; open arrows indicate SGCs which were GPR55 immunoreactive & GFAP negative (e). Stars indicate sensory neurons of different dimension, which expressed faint –to-moderate GPR55 immunoreactivity. (g–i) White arrows indicate the Neurotrace labeled nuclei of SGCs which showed PPARalpha immunoreactivity (h). Open arrow indicate autofluorescent pigment. Bar: a–i = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31608295), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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