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gp130/CD130 Antibody (28105) [Unconjugated]

 (6 Publications)
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gp130 was detected in immersion fixed paraffin-embedded sections of human pancreas tissue using Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB628) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC001) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
gp130 was detected in immersion fixed paraffin-embedded sections of human pancreas tissue using Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB628) at 5 µg/mL overnight at 4 °C. Before incubation ...read more
Recombinant Human Oncostatin M/OSM stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, B/N, ELISA(Cap)
Clone
28105
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB628-SP
Carrier Free
MAB628-500
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gp130/CD130 Antibody (28105) [Unconjugated] Summary

Immunogen
Sf 21-derived recombinant human gp130 extracellular domain
Leu24-Glu619 (Glu619-Asp, predicted)
Accession # P40189
Specificity
Detects human gp130 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) CNTF, rhIL-6, rhIL-6 R, recombinant mouse (rm) IL-6, rhIL-11, rhLIF, rhLIF R, rmLIF, or rhOSM is observed. This antibody blocks the human gp130-mediated bioactivities induced by IL-6, IL-11, LIF, OSM, and CNTF.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL6ST
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-25 ug/mL
  • Neutralization 0.02-0.1 ug/mL
Application Notes
ELISA Detection: Human gp130 Biotinylated Antibody (Catalog number BAF228)
Standard: Recombinant Human gp130 (Catalog number 228-GP)
Publications
Read Publications using
MAB628 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for gp130/CD130 Antibody (28105) [Unconjugated]

  • CD130 antigen
  • CD130
  • CDw130
  • DKFZp564F053
  • gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
  • gp130
  • IL-6 receptor subunit beta
  • IL-6R subunit beta
  • IL-6RB
  • IL-6R-beta
  • IL6ST
  • interleukin 6 signal transducer (gp130, oncostatin M receptor)
  • interleukin receptor beta chain
  • interleukin-6 receptor subunit beta
  • Interleukin-6 signal transducer
  • Membrane glycoprotein 130
  • membrane glycoprotein gp130
  • Oncostatin-M receptor subunit alpha

Background

gp130, also known as CD130, belongs to the class I cytokine receptor family. It functions as a signal transducing receptor component in the receptor complexes of the IL-6 family of cytokines including IL-6, IL-11, LIF, OSM, CNTF and CT-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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gp130/CD130
Antibody (28105) [Unc ...
MAB628
gp130/CD130 Antibody (28105) [Unconjugated]
Species: Hu
Applications: IHC, B/N, ELISA(Cap)

Publications for gp130/CD130 Antibody (MAB628)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-Fr.


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IHC-Fr
(1)
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(1)
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Showing Publications 1 - 6 of 6.
Publications using MAB628 Applications Species
Katarzyna Składanowska, Yehudi Bloch, Jamie Strand, Kerry F. White, Jing Hua, Daniel Aldridge et al. Structural basis of activation and antagonism of receptor signaling mediated by interleukin-27 Cell Reports 10/18/2022 [PMID: 36261006]
Anissa A. Widjaja, Sivakumar Viswanathan, Dong Jinrui, Brijesh K. Singh, Jessie Tan, Joyce Goh Wei Ting et al. Molecular Dissection of Pro-Fibrotic IL11 Signaling in Cardiac and Pulmonary Fibroblasts Frontiers in Molecular Biosciences 9/28/2021 [PMID: 34651016]
Jinrui Dong, Sivakumar Viswanathan, Eleonora Adami, Sebastian Schafer, Fathima F Kuthubudeen, Anissa A Widjaja et al. The pro-regenerative effects of hyperIL6 in drug-induced liver injury are unexpectedly due to competitive inhibition of IL11 signaling eLife 8/26/2021 [PMID: 34435951]
Shikha Nayar, Joshua K. Morrison, Mamta Giri, Kyle Gettler, Ling-shiang Chuang, Laura A. Walker et al. A myeloid–stromal niche and gp130 rescue in NOD2-driven Crohn’s disease Nature 5/13/2021 [PMID: 33789339]
Ludovic Zimmerlin, Tea Soon Park, Jeffrey S. Huo, Karan Verma, Sarshan R. Pather, C. Conover Talbot et al. Tankyrase inhibition promotes a stable human naïve pluripotent state with improved functionality Development 6/6/2016 [PMID: 27660325]
Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries. Mol. Hum. Reprod., 2004-03-25;10(5):313-9. 2004-03-25 [PMID: 15044601] (IHC-Fr, Human) IHC-Fr Human

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Bioinformatics

Gene Symbol IL6ST
Uniprot