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GOT2 Antibody (8B11) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
8B11
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

GOT2 Antibody (8B11) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant human GOT2 (30-430aa) purified from E.coli.
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GOT2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30067865).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for GOT2 Antibody (8B11) [CoraFluor™ 1]

  • aspartate aminotransferase, mitochondrial
  • EC 2.6.1
  • EC 2.6.1.1
  • FABP-1
  • FABPpm
  • Fatty acid-binding protein
  • FLJ40994
  • Glutamate oxaloacetate transaminase 2
  • glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2)
  • KAT4
  • KATIV
  • kynurenine aminotransferase IV
  • mAspAT
  • mitAAT
  • Plasma membrane-associated fatty acid-binding protein
  • Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GOT2 Antibody (NBP2-59428CL1) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GOT2