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Glutaminase Antibody (GT1075)

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Western Blot: Glutaminase Antibody (GT1075) [NBP3-13512] - Glutaminase (GAC) antibody detects Glutaminase (GAC) protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and ...read more
Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody (GT1075) [NBP3-13512] - Glutaminase antibody [GT1075] detects Glutaminase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were ...read more

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ICC/IF
Clone
GT1075
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Glutaminase Antibody (GT1075) Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutaminase (GAC). The exact sequence is proprietary.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GLS
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for Glutaminase Antibody (GT1075)

  • AAD20
  • EC 3.5.1.2
  • GLS
  • GLS1DKFZp686O15119
  • glutaminase C
  • glutaminase kidney isoform, mitochondrial
  • Glutaminase
  • glutaminase, phosphate-activated
  • K-Glutaminase
  • KIAA0838FLJ10358
  • L-Glutamine Amidohydrolase

Background

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GLS