I was wondering what the specificity of this enzyme is in regards to the glutaminase isoforms gls1 and gls2. Ideally, this would have no cross reactivity with gls2.

Name: Glutaminase Antibody (3211)
Brand: Novus Biologicals
SKU: NBP2-43762
Price: 449 USD

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Our Glutaminase antibody NBP2-43762 was made to an immunogen that falls between aa 551-669 of the human glutaminase C (GAC) protein: http://www.uniprot.org/uniprot/O94925#sequences. This antibody has been found to not cross react against the canonical (KGA) sequence for GLS1. It has not yet been tested for cross reactivity against the GLS2 protein. Based on an alignment of the immunogen range and the GLS2 sequence, there does seem to be some areas with significant homology, so there is a chance it could react.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, BvSpecies Glossary
Applications	WB, ICC/IF, IHC
Clone	3211
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutaminase C (GAC). The exact sequence is proprietary.
Specificity	Specific for GAC but not KGA isoform.
Predicted Species	Bovine (100%). Backed by our 100% Guarantee.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	GLS
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	No Preservative
Purity	Protein G purified

Publications using NBP2-43762	Applications	Species
Mincheva G, Gimenez-Garzo C, Izquierdo-Altarejos P et al. Golexanolone, a GABAA receptor modulating steroid antagonist, restores motor coordination and cognitive function in hyperammonemic rats by dual effects on peripheral inflammation and neuroinflammation CNS neuroscience & therapeutics 2022-07-26 [PMID: 35880480] (WB, Rat) Details: 1:1000 WB dilution	WB	Rat
Chen Y, Zhao Y, Bajor D et al. A facile and sensitive method of quantifying glutaminase binding to its inhibitor CB-839 in tissues J Genet Genomics 2020-10-02 [PMID: 33004309]
Wang, Y, Bucher, M Et al. Use of Glucose, Glutamine and Fatty Acids for Trophoblast Respiration in Lean, Obese and Gestational Diabetic Women. J Clin Endocrinol Metab 2019-05-22 [PMID: 31116396] (ICC/IF, Porcine, Bovine, Sheep, Mouse)	ICC/IF	Porcine, Bovine, Sheep, Mouse

Glutaminase Antibody (3211)

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