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Glutamate Dehydrogenase Antibody (7L7N2)

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Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat ...read more
Immunocytochemistry/ Immunofluorescence: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunofluorescence analysis of NIH-3T3 cells using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at dilution of 1:100 ...read more
Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat ...read more
Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x ...read more
Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 ...read more
Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of ...read more
Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x ...read more
Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x ...read more
Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of ...read more
Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x ...read more
Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
7L7N2
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Glutamate Dehydrogenase Antibody (7L7N2) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 459-558 of human Glutamate Dehydrogenase (P00367). ERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVHSGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTFT
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
GLUD1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Glutamate Dehydrogenase Antibody (7L7N2)

  • EC 1.4.1
  • EC 1.4.1.3
  • GDH 1
  • GDH
  • GDH1
  • GLUD
  • glutamate dehydrogenase (NAD(P)+)
  • Glutamate Dehydrogenase 1
  • glutamate dehydrogenase 1, mitochondrial
  • MGC132003

Background

One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GLUD1