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Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free

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Western Blot: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA .
Immunohistochemistry: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, ...read more
Immunohistochemistry: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C ...read more
Immunohistochemistry: Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free [NBP2-70803] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI4G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 40-315 of human GBA (NP_000148) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GBA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Theoretical MW
55.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Glucosylceramidase/GBA Antibody (OTI4G4) - Azide and BSA Free

  • Acid beta-glucosidase
  • Alglucerase
  • beta-glucocerebrosidase
  • D-glucosyl-N-acylsphingosine glucohydrolase
  • EC 3.2.1.45
  • GBA
  • GBA1
  • GC
  • GCB
  • GLUC
  • glucosidase, beta, acid
  • glucosidase, beta; acid (includes glucosylceramidase)
  • Glucosylceramidase
  • Imiglucerase
  • lysosomal glucocerebrosidase

Background

GBA encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GBA