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![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Effect of Diltiazem on L444P and N370S/V394L GC Folding and Trafficking.Western blot analysis of untreated and diltiazem-treated L444P GC cells. L444P GC cells were cultured without or with diltiazem at varying concentrations for 7 d before the cells were lysed for SDS-PAGE and Western blot analysis. GC was detected using mouse anti-GC antibody 2E2. Beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.0060026) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0019.jpg "Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Effect of Diltiazem on L444P and N370S/V394L GC Folding and Trafficking.Western blot analysis of untreated and diltiazem-treated L444P GC cells. L444P GC cells were cultured without or with diltiazem at varying concentrations for 7 d before the cells were lysed for SDS-PAGE and Western blot analysis. GC was detected using mouse anti-GC antibody 2E2. Beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.0060026) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Immunocytochemistry-Immunofluorescence-H00002629-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Immunohistochemistry-Paraffin-H00002629-M01-img0014.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - GBA monoclonal antibody (M01), clone 2E2 Analysis of GBA expression in MCF-7.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0016.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody (M01), clone 2E2.Lane 1: GBA transfected lysate(60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0017.jpg)
![ELISA: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Detection limit for recombinant GST tagged GBA is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-ELISA-H00002629-M01-img0018.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - All lanes: Western blot analysis of GBA with anti-GBA antibody [JM10-76 at 1:1,000 dilution.Lane 1: Wild-type HEK293T whole cell lysate (20 ug).Lane 2: GBA knockout HEK293T whole cell lysate (20 ug). NBP2-66871was shown to specifically react with GBA in wild-type HEK293T cells. No band was observed when GBA knockout sample was tested. Wild-type and GBA knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH , 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Western-Blot-NBP2-66871-img0011.jpg "Western Blot: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - All lanes: Western blot analysis of GBA with anti-GBA antibody [JM10-76 at 1:1,000 dilution.Lane 1: Wild-type HEK293T whole cell lysate (20 ug).Lane 2: GBA knockout HEK293T whole cell lysate (20 ug). NBP2-66871was shown to specifically react with GBA in wild-type HEK293T cells. No band was observed when GBA knockout sample was tested. Wild-type and GBA knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH , 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded mouse pancreas tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0015.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Western blot analysis of Glucosylceramidase/GBA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: A549 cell lysate Lane 3: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Western-Blot-NBP2-66871-img0009.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0001.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human liver tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0003.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human pancreas tissue using anti-GBA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0004.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded human kidney tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded rat liver tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0013.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded rat kidney tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-JM10-76-Immunohistochemistry-Paraffin-NBP2-66871-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Glucosylceramidase/GBA Antibody [NBP1-32271] - Analysis of methanol-fixed HeLa, using GBA antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32271-img0010.jpg "Immunocytochemistry/Immunofluorescence: Glucosylceramidase/GBA Antibody [NBP1-32271] - Analysis of methanol-fixed HeLa, using GBA antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-32271] - Lung cancer, using GBA antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-Immunohistochemistry-Paraffin-NBP1-32271-img0008.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - Sample(30 ug of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-Western-Blot-NBP1-32271-img0013.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - MCF-7 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucosylceramidase/GBA antibody [C1C3] (NBP1-32271) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32271_rabbit-polyclonal-glucosylceramidase-gba-antibody-91020242013402.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Glucosylceramidase/GBA Antibody [NBP1-32271] - Wild-type (WT) and GBA knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32271_rabbit-polyclonal-glucosylceramidase-gba-antibody-1610202419414552.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (4G4) [NBP2-45829] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA .](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-4G4-Western-Blot-NBP2-45829-img0005.jpg "Western Blot: Glucosylceramidase/GBA Antibody (4G4) [NBP2-45829] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA .")
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (4G4) [NBP2-45829] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-4G4-Immunohistochemistry-NBP2-45829-img0004.jpg)
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (4G4) [NBP2-45829] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-4G4-Immunohistochemistry-NBP2-45829-img0001.jpg)
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (4G4) [NBP2-45829] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-4G4-Immunohistochemistry-NBP2-45829-img0002.jpg)
![Immunohistochemistry: Glucosylceramidase/GBA Antibody (4G4) [NBP2-45829] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-4G4-Immunohistochemistry-NBP2-45829-img0003.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody [H00002629-B01P] - Analysis of GBA expression in U-2 OS.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-Western-Blot-H00002629-B01P-img0003.jpg "Western Blot: Glucosylceramidase/GBA Antibody [H00002629-B01P] - Analysis of GBA expression in U-2 OS.")
![Western Blot: Glucosylceramidase/GBA Antibody [H00002629-B01P] - Analysis of GBA expression in transfected 293T cell line by GBA polyclonal antibody. Lane 1: GBA transfected lysate(58.96 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-Western-Blot-H00002629-B01P-img0004.jpg)
![Enzyme Activity: Glucosylceramidase/GBA Colorimetric Assay Kit [KA1611] - Kinetics of beta-glucosidase reaction in 96-well plate assay.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Colorimetric-Assay-Kit-Enzyme-Activity-KA1611-img0001.jpg "Enzyme Activity: Glucosylceramidase/GBA Colorimetric Assay Kit [KA1611] - Kinetics of beta-glucosidase reaction in 96-well plate assay.")
![Western Blot: Glucosylceramidase/GBA Antibody (8O3Z7) [NBP3-15637] - Western blot analysis of extracts of various cell lines, using Glucosylceramidase beta (Glucosylceramidase/GBA) antibody (NBP3-15637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-8O3Z7-Western-Blot-NBP3-15637-img0002.jpg "Western Blot: Glucosylceramidase/GBA Antibody (8O3Z7) [NBP3-15637] - Western blot analysis of extracts of various cell lines, using Glucosylceramidase beta (Glucosylceramidase/GBA) antibody (NBP3-15637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (8O3Z7) [NBP3-15637] - Immunohistochemistry of paraffin-embedded human liver using Glucosylceramidase beta (Glucosylceramidase/GBA) Rabbit mAb (NBP3-15637) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-8O3Z7-Immunohistochemistry-Paraffin-NBP3-15637-img0001.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (8O3Z7) [NBP3-15637] - Western blot analysis of extracts of Rat brain, using Glucosylceramidase beta (Glucosylceramidase/GBA) antibody (NBP3-15637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-8O3Z7-Western-Blot-NBP3-15637-img0003.jpg)
![Western Blot: GBA Overexpression Lysate (Adult Normal) [NBL1-10990] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GBA.](http://images.novusbio.com/fullsize/GBA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10990-img0002.jpg "Western Blot: GBA Overexpression Lysate (Adult Normal) [NBL1-10990] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GBA.")
![Western Blot: GBA Overexpression Lysate (Adult Normal) [NBL1-10989] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GBA.](http://images.novusbio.com/fullsize/GBA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10989-img0002.jpg "Western Blot: GBA Overexpression Lysate (Adult Normal) [NBL1-10989] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GBA.")
![Western Blot: GBA Overexpression Lysate (Adult Normal) [NBP2-08846] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GBA.](http://images.novusbio.com/fullsize/GBA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08846-img0001.jpg "Western Blot: GBA Overexpression Lysate (Adult Normal) [NBP2-08846] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GBA.")
![Western Blot: GBA Overexpression Lysate (Adult Normal) [NBP2-09097] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GBA.](http://images.novusbio.com/fullsize/GBA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09097-img0001.jpg "Western Blot: GBA Overexpression Lysate (Adult Normal) [NBP2-09097] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GBA.")
![Western Blot: Glucosylceramidase/GBA Antibody (4H4) [NBP3-26559] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, U87 whole cell lysate. All lanes: Glucosylceramidase/GBA Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 58, 55, 51, 30 kDa Observed band size: 60 kDa](http://images.novusbio.com/fullsize/nbp3-26559_rabbit-glucosylceramidase-gba-mab-4h4-282202411485832.jpg "Western Blot: Glucosylceramidase/GBA Antibody (4H4) [NBP3-26559] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, U87 whole cell lysate. All lanes: Glucosylceramidase/GBA Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 58, 55, 51, 30 kDa Observed band size: 60 kDa")