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GLI-3 Antibody (2352B) [Unconjugated]

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GLI‑3 was detected in immersion fixed  SW480 fibroblast carcinoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Rabbit Anti-Human GLI‑3 Monoclonal Antibody (Catalog # MAB3690) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GLI‑3 was detected in immersion fixed  SW480 fibroblast carcinoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Rabbit Anti-Human GLI‑3 Monoclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
2352B
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3690-SP
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GLI-3 Antibody (2352B) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E. coli-derived recombinant human GLI-3
Met1-Glu479
Accession # P10071
Specificity
Detects human GLI-3 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLI-3 Antibody (2352B) [Unconjugated]

  • ACLS
  • ACLSPAPAPHS
  • ADD
  • Bph
  • GCPS
  • GLI family zinc finger 3
  • GLI3 form of 190 kDa
  • GLI3 full length protein
  • GLI3
  • GLI-3
  • GLI3-190
  • GLI3FL
  • GLI-Kruppel family member GLI3
  • glioma-associated oncogene family zinc finger 3
  • Greig cephalopolysyndactyly syndrome
  • oncogene GLI3
  • PAP-A
  • PAPA1
  • PAPB
  • PAPBDNA-binding protein
  • Pdn
  • PHS
  • PPDIV
  • Xt
  • zinc finger protein GLI3

Background

GLI3 is a zinc finger protein.  The GLI family of proteins are transcription factors and are mediators of Sonic hedgehog signaling.  GLI3 is known to be a transcriptional repressor but also may have a positive transcription function. Mutations in the GLI3 gene are associated with many polydactyly diseases.

Rupert JM., Vogelstein B., Arheden K., Kinzler KW., "GLI3 Encodes a 190-kilodalton Protein with Multiple Regions of GLI Similarity". Molecular and Cellular Biology. 1990 Oct; 10(10):  5408-15. Taipale J., Beachy PA., "The Hedgehog and Wnt Signaling Pathways in Cancer".  Nature.  2001 May;411(6835):  349-54. Rash BG., Grove EA., "Patterning the Dorsal Telencephalon: a Role for Sonic Hedgehog ?".  The Journal of Neuroscience.  2007 Oct; 27(43):  11595-603.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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