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GLI-3 Antibodies

We offer GLI-3 Antibodies for use in common research applications: Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each GLI-3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GLI-3 Antibodies can be used in a variety of model species: Canine, Human, Mouse, Rat. Use the list below to choose the GLI-3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GLI-3 polyclonal antibodies and browse our GLI-3 monoclonal antibody catalog.

Alternate Names for GLI-3 Antibodies

anti-GLI-3 antibody, anti-GLI3 antibody, anti-ACLSPAPAPHS antibody, anti-GCPS antibody, anti-GLI family zinc finger 3 antibody, anti-GLI-Kruppel family member GLI3 antibody, anti-GLI3 form of 190 kDa antibody, anti-GLI3 full length protein antibody, anti-GLI3-190 antibody, anti-GLI3FL antibody, anti-glioma-associated oncogene family zinc finger 3 antibody, anti-Greig cephalopolysyndactyly syndrome antibody, anti-oncogene GLI3 antibody, anti-PAP-A antibody, anti-PAPA1 antibody, anti-PAPBDNA-binding protein antibody, anti-PPDIV antibody, anti-zinc finger protein GLI3 antibody

9 Results for "gli-3" in Primary Antibodies

AF3690

GLI-3 Antibody

(3 Reviews)

(102 Publications)

100 μg

AF3690

Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GLI-3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI‑3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The gli-1 promoter was detected by standard PCR.

GLI-3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Conditional deletion of GATA6 does not significantly alter Gli3 processing in the hindlimb bud.(A) Western blot analysis of Gli3FL and Gli3R steady state levels in anterior (lanes 1 and 3) or posterior (lanes 2 and 4) halves of E11.5 hindlimb buds isolated from either control (lanes 1 and 2) or Prx1-Cre; GATA6fl/fl (lanes 3 and 4) mouse embryos. alpha –Tubulin is used as a loading control. (B) Quantification of Gli3FL/Gli3R ratios (by densitometry of Western Blots) in anterior or posterior halves of E11.5 hindlimb buds isolated from either littermate controls (lanes 1 and 2) or Prx1-Cre; GATA6fl/fl (lanes 3 and 4) animals, 12 embryos of each genotype were analyzed. Error bar indicates standard deviation, n = 12. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24415953), licensed under a CC-BY license. Not internally tested by R&D Systems.

SufuT396I does not stabilize Gli3FL protein and reduces the processing of Gli3FL.(A) Western blotting of lysates prepared from SufuR146X/R146X at E9.5 and SufuT396I/T396I, SufuT396I/+, and wild-type embryos at E10.5 with anti-Gli3, anti-Sufu, and anti-actin antibodies. Each image presented in the Fig. is a representative of independent triplicated experiments. The full gel images are shown in S6A Fig. (B, C) Relative expression of Gli3FL (B) and Gli3REP (B and C). Western blotting was performed two times using lysates prepared from five wild-type and five SufuT396I/T396I embryos at E10.5 (S2A Fig.). Expression levels were quantified from the band intensity shown in S2A Fig. as relative values of the Gli3FL/actin and Gli3REP/actin expression ratios (B) and the direct ratio of Gli3REP/Gli3FL (C). (**) p < 0.01, two-tailed Student’s t-test. Error bars indicate the standard deviations. (D) Western blotting of cell lysates from wild-type and SufuT396I/T396I MEFs with indicated antibodies. The SufuT396I/T396I MEFs were electroporated with 10.0, 1.0, or 0.1 μg of the HA–Sufu construct (lane 3, 4, and 5, respectively), or 10 μg of the HA–SufuT396I construct (lane 6). The mobilities on SDS-PAGE of the wild-type Sufu (lane 3–5) and SufuT396I (lane 6) are identical. The complete gel images are shown in S6B Fig. This image is representative of two independent experiments. (E) Western blotting of cell lysates from Sufu−/− cells with indicated antibodies. The Sufu−/− cells were electroporated with a mixture of the Flag–Gli3 construct (6 μg) and the HA–Sufu construct (4.00, 1.33, 0.44, or 0.15 μg) for the wild-type Sufu cotransfection (lane 2 to 5, respectively), or a mixture of the Flag–Gli3 construct (6 μg) and the HA–SufuT396I construct (4 μg) for mutant Sufu cotransfection (lane 6). The complete gel images are shown in S6C Fig. This image is representative of two independent experiments. (F) Western blotting of immunoprecipitates or lysates from 293T cells transfected with expression constructs as indicated at the top. The complete gel images are shown in S6D Fig. This image is representative of two independent experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25760946), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF3690

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3690-SP

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NBP2-29627

GLI-3 Antibody - BSA Free

(1 Review)

(3 Publications)

0.1 mg

NBP2-29627

Western Blot: GLI-3 Antibody [NBP2-29627] - Adult mouse brain tissue lysates. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: GLI-3 Antibody [NBP2-29627] - HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-29627] - Analysis of in mouse liver.

Flow (Intracellular): GLI-3 Antibody [NBP2-29627] - Intracellular staining for GLI-3 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-3 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.

Western Blot: GLI-3 Antibody [NBP2-29627] - Analysis of GLI-3 in Hela cell lysate and MEF cell lysate.

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-29627] - Analysis of in mouse testis.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-29627AF350
Alexa Fluor 405	NBP2-29627AF405
Alexa Fluor 488	NBP2-29627AF488
Alexa Fluor 532	NBP2-29627AF532
Alexa Fluor 594	NBP2-29627AF594
Alexa Fluor 647	NBP2-29627AF647
Alexa Fluor 700	NBP2-29627AF700
Alexa Fluor 750	NBP2-29627AF750
Allophycocyanin	NBP2-29627APC
Biotin	NBP2-29627B
CoraFluor 1	NBP2-29627CL1
DyLight 350	NBP2-29627UV
DyLight 405	NBP2-29627V
DyLight 488	NBP2-29627G
DyLight 550	NBP2-29627R
DyLight 594	NBP2-29627DL594
DyLight 650	NBP2-29627C
DyLight 680	NBP2-29627FR
DyLight 755	NBP2-29627IR
FITC	NBP2-29627F
HRP	NBP2-29627H
Janelia Fluor 525	NBP2-29627JF525
Janelia Fluor 549	NBP2-29627JF549
Janelia Fluor 646	NBP2-29627JF646
Janelia Fluor 669	NBP2-29627JF669
PE	NBP2-29627PE
PerCP	NBP2-29627PCP
mFluor Violet 450 SE	NBP2-29627MFV450
mFluor Violet 500 SE	NBP2-29627MFV500
mFluor Violet 610 SE	NBP2-29627MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-29627SS

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NBP2-16665

GLI-3 Antibody

0.1 ml

NBP2-16665

Western Blot: GLI-3 Antibody [NBP2-16665] - A: Jurkat.

Immunocytochemistry/Immunofluorescence: GLI-3 Antibody [NBP2-16665] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-16665] - Human lung adenocarcinoma. GLI3 antibody diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: GLI-3 Antibody [NBP2-16665] - A. PC-12 whole cell lysate/extract B. Rat whole cell lysate/extract.

Western Blot: GLI-3 Antibody [NBP2-16665] - A: mouse ESC.

Reactivity:

Hu, Mu, Rt, Ch, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-16166

GLI-3 Antibody (8B1E2)

100 ul

NBP3-16166

Western Blot: GLI-3 Antibody (8B1E2) [NBP3-16166] - Western blot analysis of extracts of HeLa cells, using GLI-3 antibody (NBP3-16166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunocytochemistry/Immunofluorescence: GLI-3 Antibody (8B1E2) [NBP3-16166] - Immunofluorescence analysis of U-2 OS cells using GLI-3 Rabbit mAb (NBP3-16166) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: GLI-3 Antibody (8B1E2) [NBP3-16166] - Immunofluorescence analysis of C6 cells using GLI-3 Rabbit mAb (NBP3-16166) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB3690

GLI-3 Antibody (2352B) [Unconjugated]

100 μg

MAB3690

GLI‑3 was detected in immersion fixed SW480 fibroblast carcinoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Rabbit Anti-Human GLI‑3 Monoclonal Antibody (Catalog # MAB3690) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3690-SP

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H00002737-M01

GLI-3 Antibody (2C9)

0.1 mg

H00002737-M01

Immunocytochemistry/Immunofluorescence: GLI-3 Antibody (2C9) [H00002737-M01] - Analysis of monoclonal antibody to GLI3 on HeLa cell . Antibody concentration 10 ug/ml.

Proximity Ligation Assay: GLI-3 Antibody (2C9) [H00002737-M01] - Analysis of protein-protein interactions between SMAD1 and GLI3. HeLa cells were stained with anti-SMAD1 rabbit purified polyclonal 1:1200 and anti-GLI3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

ELISA: GLI-3 Antibody (2C9) [H00002737-M01] - Detection limit for recombinant GST tagged GLI3 is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-89275

GLI-3 Antibody

0.1 ml

NBP1-89275

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275] - Staining of human pancreas shows no nuclear positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275] - Staining of human spleen shows moderate to strong nuclear positivity in cells in red pulp.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP3-20039

GLI-3 Antibody (S08-2B9)

100 ul

NBP3-20039

Western Blot: GLI-3 Antibody (S08-2B9) [NBP3-20039] - Western blot detection of GLI-3 in 293 cell lysates using NBP3-20039 (1:1000 diluted). Predicted band size: 170kDa. Observed band size: 200kDa.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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BAF3690

GLI-3 Antibody [Biotin]

50 μg

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Biotin

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3690
Carrier Free	AF3690-SP

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