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GLI-1 Antibody (1B9F8) - BSA Free

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Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against human GLI1 (AA: 284-449) recombinant protein. (Expected MW is 43.1 kDa)
Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against human GLI1 (AA: 284-449) recombinant protein. (Expected MW is 43.1 kDa)
Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of Hela cells using GLI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of esophageal cancer tissues using GLI1 mouse mAb with DAB staining.
Flow Cytometry: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of HepG2 cells using GLI1 mouse mAb (green) and negative control (red).
Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against HEK293 (1) and GLI1 (AA: 284-449)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of ovarian cancer tissues using GLI1 mouse mAb with DAB staining.
ELISA: GLI-1 Antibody (1B9F8) [NBP2-52475] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
1B9F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52475-0.025mg
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GLI-1 Antibody (1B9F8) - BSA Free Summary

Immunogen
Purified recombinant fragment of human GLI-1 (AA: 284-449) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GLI1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for GLI-1 Antibody (1B9F8) - BSA Free

  • GLI family zinc finger 1
  • GLI1
  • GLI-1
  • glioma-associated oncogene family zinc finger 1
  • glioma-associated oncogene homolog 1 (zinc finger protein)
  • Glioma-associated oncogene
  • GLIzinc finger protein GLI1
  • Oncogene GLI

Background

Gli1 was produced against a peptide corresponding to the carboxy-terminal region of the mouse Gli-1 protein. This region is not conserved among other gli family members, namely Gli-2 and Gli-3. Gli was termed by Kinzler et al. (1987) as 'glioma-associated oncogene' amplified in malignant gliomas. Analysis of the cloned gene demonstrates that the gene contains 5 repeats of zinc-finger sequences, which places gli in the family of Kruppel (Kr) zinc finger proteins. Northern analysis reveals that GLI is expressed in embryonal carcinoma cells but not in most adult tissue. GLI has been localized to 12q13-q14.3 by Southern blot analysis. On mouse the gene is located on chromosome 10. In mice, 3 zinc finger transcription factors, Gli-1, Gl-i2 and Gli-3, have been implicated in the transduction of Sonic hedgehog (Shh) signal. In papillary epithelium, shh, gli1 and ptc all follow similar expression patterns. Gli1 expression is central and probably sufficient for tumor development in humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GLI1