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Rat Gelsolin/GSN ELISA Kit (Colorimetric)

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ELISA: Rat Gelsolin/GSN ELISA Kit (Colorimetric) [NBP2-82211] - Samples were spiked with high concentrations of Rat Gelsolin/GSN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Gelsolin/GSN ELISA Kit (Colorimetric) [NBP2-82211] - Samples were spiked with high concentrations of Rat Gelsolin/GSN and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Rat Gelsolin/GSN ELISA Kit (Colorimetric) [NBP2-82211] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
15.63 - 1000 ng/mL
Sensitivity
18.75 ng/mL

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Rat Gelsolin/GSN ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Gelsolin/GSN in samples. No Significant cross-reactivity or interference between Rat Gelsolin/GSN and analogues was observed.
Standard Curve Range
15.63 - 1000 ng/mL
Sensitivity
18.75 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 11%
Intra-Assay
CV% < 10%
Spike Recovery
average = 94% for serum; average = 98% for EDTA plasma; average = 93% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GSN

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Rat Gelsolin/GSN ELISA Kit (Colorimetric)

  • Actin-depolymerizing factor
  • ADF
  • AGEL
  • Brevin
  • DKFZp313L0718
  • gelsolin (amyloidosis, Finnish type)
  • Gelsolin
  • GSN

Background

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GSN