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Gelsolin/GSN Antibody

Images

 
Western Blot: Gelsolin/GSN Antibody [NB100-53814] - Staining of Human Colorectal cancer lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Flow Cytometry: Gelsolin/GSN Antibody [NB100-53814] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Gelsolin/GSN Antibody Summary

Immunogen
Peptide with sequence C-PRLKDKKMDAHP corresponding to internal region according to NP_937895.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_000168.1 and NP_937895.1).
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GSN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 2 ug/mL
Publications
Read Publication using NB100-53814.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Gelsolin/GSN Antibody

  • Actin-depolymerizing factor
  • ADF
  • AGEL
  • Brevin
  • DKFZp313L0718
  • gelsolin (amyloidosis, Finnish type)
  • Gelsolin
  • GSN

Background

Gelsolin is a calcium dependent actin binding protein. It is a potent modulator of actin filament length and gelation. Gelsolin has been shown to exist in at least two variant forms, cytoplasmic gelsolin and plasma gelsolin. Plasma gelsolin has also been called actin depolymerizing factor (ADF) or brevin. Human plasma and rabbit macrophage gelsolins differ by the presence of a 25 amino acid residue extension at the NH2 terminus in the human plasma gelsolin which appears to account for the difference in relative molecular weights.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Gelsolin/GSN Antibody (NB100-53814)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GSN
Entrez
Uniprot