![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (336) diluted at 1:500. HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0007.jpg "Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (336) diluted at 1:500. HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | 336 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ARHGEF2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for GEF-H1 Antibody (NBP2-43635)Find related products by research area.
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| Gene Symbol | ARHGEF2 |
![Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: GEF-H1 protein stained by GEF-H1 antibody [336] diluted at 1:50. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Immunocytochemistry-Immunofluorescence-NBP2-43635-img0006.jpg "Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: GEF-H1 protein stained by GEF-H1 antibody [336] diluted at 1:50. Blue: Hoechst 33342 staining.")
![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract 7.5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0001.jpg "Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract 7.5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500")
![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug C8D30 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0002.jpg "Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug C8D30 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500")
![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0003.jpg "Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500.")
![Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of Sample: MDA-MB-231 cells were fixed in methanol for 5 min. Green: GEF-H1 protein stained by GEF-H1 antibody [336] diluted at 1:50.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Immunocytochemistry-Immunofluorescence-NBP2-43635-img0004.jpg "Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of Sample: MDA-MB-231 cells were fixed in methanol for 5 min. Green: GEF-H1 protein stained by GEF-H1 antibody [336] diluted at 1:50.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")