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GATA-6 Antibody [Unconjugated]

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Western blot shows cytoplasmic and nuclear extracts from PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
GATA-6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) for 3 hours at room temperature. ...read more
KATO-III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA-6/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human GATA-6 ...read more
GATA-6 was detected in immersion fixed paraffin-embedded sections of human intestine using Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) at 5 µg/mL overnight at 4 °C. Tissue ...read more
Simple Western shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.2 mg/ml. A specific band was detected for GATA‑6 at approximately 62 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ...read more
p38-Mapk14/11 inhibition during blastocyst maturation blocks PrE differentiation/maturation. (a) Experimental schema of p38-Mapk14/11 inhibition (+SB220025), plus vehicle control (+DMSO), and the details of antibodies ...read more
Reduced expression of the primitive endoderm markers Gata4 and Gata6 and increased expression of Nanog was observed in embryoid bodies following inhibitor of the Fgfr.Embryoid bodies were grown in different ...read more
Reduced expression of primitive endoderm markers Gata4 and Gata6 and increased expression of Nanog was observed in embryoid bodies upon inhibition of Mek.Embryoid bodies were grown in different concentrations of ...read more
Reduced expression of the primitive endoderm markers Gata4 and Gata6 and increased expression of Nanog was observed in embryoid bodies following inhibitor of the Fgfr.Embryoid bodies were grown in different ...read more
expression of primitive endoderm cell fate markers gradually increased and nanog decreased in developing embryoid bodies.Embryoid bodies were produced from R63 mES cells using the hanging drop method. Development of the ...read more
Gata factor expression in embryoid bodies (EBs) at day 5.5 culture and in ex vivo visceral yolk sac (VYS) at E17.5 in relation to maternal diet. (A) Expression of Gata4, Gata6 and Dab2 mRNA in EBs of Emb-LPD and NPD ...read more
Reduced expression of primitive endoderm markers Gata4 and Gata6 and increased expression of Nanog was observed in embryoid bodies upon inhibition of Mek.Embryoid bodies were grown in different concentrations of ...read more
Gata factor expression in embryoid bodies (EBs) at day 5.5 culture and in ex vivo visceral yolk sac (VYS) at E17.5 in relation to maternal diet. (A) Expression of Gata4, Gata6 and Dab2 mRNA in EBs of Emb-LPD and NPD ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

GATA-6 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-6
Met1-Thr449
Accession # Q92908
Specificity
Detects human GATA-6 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA6
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 6 Reviews rated 4
using
AF1700 in the following applications:

Publications
Read Publications using
AF1700 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-6 Antibody [Unconjugated]

  • GATA binding protein 6
  • GATA6
  • GATA-6
  • GATA-binding factor 6
  • GATA-binding protein 6
  • transcription factor GATA-6

Background

GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-6 Antibody (AF1700)(139)

We have publications tested in 6 confirmed species: Human, Mouse, Bovine, Cynomolgus Monkey, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Transgenic Mouse.

We have publications tested in 12 applications: ChIP, Flow Cytometry, ICC, ICC/IF, IF/ICC, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(4)
Flow Cytometry
(5)
ICC
(33)
ICC/IF
(1)
IF/ICC
(1)
IHC
(21)
IHC-Fr
(3)
IHC-P
(1)
Immunocytochemistry
(8)
Immunohistochemistry
(7)
Immunoprecipitation
(2)
Western Blot
(6)
All Applications
Filter By Species
Human
(26)
Mouse
(46)
Bovine
(2)
Cynomolgus Monkey
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 139. Show All 139 Publications.
Publications using AF1700 Applications Species
Ming, H;Scatolin, GN;Ojeda, A;Jiang, Z; Establishment of bovine extraembryonic endoderm cells bioRxiv : the preprint server for biology 2024-12-17 [PMID: 39763779] (Immunocytochemistry, Bovine) Immunocytochemistry Bovine
Nicoletti, C;Massenet, J;Pintado-Urbanc, AP;Connor, LJ;Nicolau, M;Sundar, S;Xu, M;Schmitt, A;Zhang, W;Fang, Z;Chan, TCI;Tapscott, SJ;Cheung, TH;Simon, MD;Caputo, L;Puri, PL; E-box independent chromatin recruitment turns MYOD into a transcriptional repressor bioRxiv : the preprint server for biology 2024-12-05 [PMID: 39677796] (Immunocytochemistry, Human) Immunocytochemistry Human
Pinzon-Arteaga, CA;O'Hara, R;Mazzagatti, A;Ballard, E;Hu, Y;Pan, A;Schmitz, DA;Wei, Y;Sakurai, M;Ly, P;Banaszynski, LA;Wu, J; TASOR expression in naive embryonic stem cells safeguards their developmental potential Cell reports 2024-10-24 [PMID: 39453814] (Immunohistochemistry, Human) Immunohistochemistry Human
McCreery, KP;Stubb, A;Stephens, R;Fursova, NA;Cook, A;Kruse, K;Michelbach, A;Biggs, LC;Keikhosravi, A;Nykänen, S;Hydén-Granskog, C;Zou, J;Lackmann, JW;Niessen, CM;Vuoristo, S;Miroshnikova, YA;Wickström, SA; Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells bioRxiv : the preprint server for biology 2024-09-07 [PMID: 39372762] (Immunohistochemistry, Immunocytochemistry, Human) Immunohistochemistry, Immunocytochemistry Human
Gutierrez-Sainz, L;Heredia-Soto, V;Rodríguez-García, AM;Crespo Sánchez, MG;Serrano-Olmedo, MG;Molero-Luis, M;Losantos-García, I;Ghanem, I;Pérez-Wert, P;Custodio, A;Mendiola, M;Feliu, J; Cytokines and Pancreatic Ductal Adenocarcinoma: Exploring Their Relationship with Molecular Subtypes and Prognosis International journal of molecular sciences 2024-08-29 [PMID: 39273323] (Immunohistochemistry, Human) Immunohistochemistry Human
Schumacher, S;Fernkorn, M;Marten, M;Chen, R;Kim, YS;Bedzhov, I;Schröter, C; Tissue-intrinsic beta-catenin signals antagonize Nodal-driven anterior visceral endoderm differentiation Nature communications 2024-06-13 [PMID: 38871742] (Immunocytochemistry, Human) Immunocytochemistry Human
Dattani, A;Corujo-Simon, E;Radley, A;Heydari, T;Taheriabkenar, Y;Carlisle, F;Lin, S;Liddle, C;Mill, J;Zandstra, PW;Nichols, J;Guo, G; Naive pluripotent stem cell-based models capture FGF-dependent human hypoblast lineage specification Cell stem cell 2024-05-24 [PMID: 38823388] (Immunohistochemistry, Human) Immunohistochemistry Human
Tan, JP;Liu, X;Polo, JM; Reprogramming fibroblast into human iBlastoids Nature protocols 2024-04-17 [PMID: 38632379] (Immunocytochemistry, Human) Immunocytochemistry Human
Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M; Distinct pathways drive anterior hypoblast specification in the implanting human embryo Nature cell biology 2024-03-01 [PMID: 38443567] (Immunohistochemistry, Human, Mouse) Immunohistochemistry Human, Mouse
Khoa, LTP;Yang, W;Shan, M;Zhang, L;Mao, F;Zhou, B;Li, Q;Malcore, R;Harris, C;Zhao, L;Rao, R;Iwase, S;Kalantry, S;Bielas, SL;Lyssiotis, CA;Dou, Y; Quiescence enables unrestricted cell fate in naive embryonic stem cells Nature communications 2024-02-26 [PMID: 38409226] (Immunocytochemistry, Mouse) Immunocytochemistry Mouse
Show All 139 Publications.

Reviews for GATA-6 Antibody (AF1700) (6) 46

Average Rating: 4
(Based on 6 reviews)
We have 6 reviews tested in 1 species: Mouse.

Reviews using AF1700:
Filter by Applications
ICC
(1)
IHC-P
(2)
IF
(2)
ChIP
(1)
All Applications
Filter by Species
Mouse
(6)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry GATA-6 AF1700
Enlarge
4
reviewed by:
Verified Customer
ICC Mouse 08/04/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotKWT0214121
  5
reviewed by:
Verified Customer
IHC-P Mouse 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23002247
SpeciesMouse
  4
reviewed by:
Verified Customer
IHC-P Mouse 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22733991
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22733991
SpeciesMouse
  4
reviewed by:
Verified Customer
ChIP Mouse 01/05/2015
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedSee PMID 23002247
SpeciesMouse
  3
reviewed by:
Verified Customer
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 20826529
SpeciesMouse

Product General Protocols

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FAQs for GATA-6 Antibody (AF1700) (0)

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Recent Reviews

4
5
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Verified Customer
08/04/2016
Application: ICC
Species: Mouse

 
Verified Customer
01/05/2015
Application: IHC-P
Species: Mouse

 
Verified Customer
01/05/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol GATA6
Uniprot