![SDS-Page: Recombinant Human GAPDH Protein [NBP2-52615] - Observed molecular weight: 36 kDa](http://images.novusbio.com/fullsize/Recombinant-Human-GAPDH-Protein-SDS-Page-NBP2-52615-img0002.jpg "SDS-Page: Recombinant Human GAPDH Protein [NBP2-52615] - Observed molecular weight: 36 kDa")
| Reactivity | HuSpecies Glossary |
| Applications | PAGE, Enzyme Activity |
| Concentration | 1 mg/ml |
| Description | An un-tagged recombinant protein corresponding to the amino acids 1-335 of Human GAPDH Source: E.coli Amino Acid Sequence: MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE |
| Details of Functionality | Specific activity is > 50 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | GAPDH |
| Purity | >95%, by SDS-PAGE |
| Dilutions |
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| Theoretical MW | 36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 1 mM EDTA, 20% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | >95%, by SDS-PAGE |
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Research Areas for GAPDH Recombinant Protein (NBP2-52615)Find related products by research area.
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The use of Beta Actin (AC-15) as a loading control across multiple species Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs. Actin has six highly conserved isoforms, however beta and gamma actin are the two... Read full blog post. |
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Tips on choosing an ideal loading control antibody for Western Blotting Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p... Read full blog post. |
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GAPDH - A "Housekeeping" Gene With Diverse Functions in Cellular Homeostasis Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a well-known housekeeping gene with functions in glycolysis. Many biologists are familiar with the gene and use GAPDH antibodies for a loading control when performing western blots. However, this ... Read full blog post. |
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A New Standard in Antibody Testing - Simple Western Certified Antibodies The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi... Read full blog post. |
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GAPDH: More than a housekeeping gene GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway. Apart from its role in gl... Read full blog post. |
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GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase) GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin... Read full blog post. |
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Time to Start Actin Like a Reliable 'Housekeeper'! A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti... Read full blog post. |
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Beta Actin and GAPDH: The Importance of Western Blot Loading Controls What are Western Blot Loading Controls?Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use... Read full blog post. |
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The GAPDH Antibody in Western Blot Assays The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch... Read full blog post. |
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