![Immunohistochemistry-Paraffin: GAP-43 Antibody [Biotin] [NB300-143B] - Representative images of hematoxylin and eosin. Differences in the number of OMP+ mature olfactory receptor neurons (ORNs). Image collected and cropped by Citeab from the following publication (Reduction of Proliferating Olfactory Cells and Low Expression of Extracellular Matrix Genes Are Hallmarks of the Aged Olfactory Mucosa. Front Aging Neurosci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GAP-43-Antibody-[Biotin]-Immunohistochemistry-Paraffin-NB300-143B-img0001.jpg "Immunohistochemistry-Paraffin: GAP-43 Antibody [Biotin] [NB300-143B] - Representative images of hematoxylin and eosin. Differences in the number of OMP+ mature olfactory receptor neurons (ORNs). Image collected and cropped by Citeab from the following publication (Reduction of Proliferating Olfactory Cells and Low Expression of Extracellular Matrix Genes Are Hallmarks of the Aged Olfactory Mucosa. Front Aging Neurosci (2018)) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Eq, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Biotin |
| Description | This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet. |
| Immunogen | C-terminal peptide of rodent GAP43, KEDPEADQEHA coupled to KLH. |
| Localization | Neuronal Marker, developing and regenerating axons and growth cone. |
| Marker | Neuronal Marker |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GAP43 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This GAP43 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded and frozen sections and Western blot, where it recognizes a band at 43 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Readout System | ||
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| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for GAP-43 Antibody (NB300-143B)Find related products by research area.
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Synapsin I: Implicated in synaptic activity across a diverse range of studies Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal. Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i... Read full blog post. |
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| Gene Symbol | GAP43 |
![Immunohistochemistry-Paraffin: GAP-43 Antibody [Biotin] [NB300-143B] - Immunohistochemistry-Paraffin: GAP-43 Antibody [Biotin] [NB300-143B] - Representative images of immunohistological staining (brown) of OMP-positive (OMP+) cells, GAP43+ immature ORNs. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Numbers of SOX2+ ORN progenitors and Ki67+ actively proliferating cells per mm of the basal layer and OMP+ mature ORNs, GAP43+ immature ORNs, and Cas3+ apoptotic cells per mm of the OE in saline or rhIGF-1-treated mice. Open circles, rectangles, and triangles represent the values for each mouse in the saline, low-IGF-1, and high-IGF-1 treated groups (each n = 6), respectively. Image collected and cropped by Citeab from the following publication (Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium. Front Aging Neurosci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GAP-43-Antibody-[Biotin]-Immunohistochemistry-Paraffin-NB300-143B-img0002.jpg "Immunohistochemistry-Paraffin: GAP-43 Antibody [Biotin] [NB300-143B] - Immunohistochemistry-Paraffin: GAP-43 Antibody [Biotin] [NB300-143B] - Representative images of immunohistological staining (brown) of OMP-positive (OMP+) cells, GAP43+ immature ORNs. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Numbers of SOX2+ ORN progenitors and Ki67+ actively proliferating cells per mm of the basal layer and OMP+ mature ORNs, GAP43+ immature ORNs, and Cas3+ apoptotic cells per mm of the OE in saline or rhIGF-1-treated mice. Open circles, rectangles, and triangles represent the values for each mouse in the saline, low-IGF-1, and high-IGF-1 treated groups (each n = 6), respectively. Image collected and cropped by Citeab from the following publication (Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium. Front Aging Neurosci (2018)) licensed under a CC-BY license.")
![Immunohistochemistry: GAP-43 Antibody [Biotin] [NB300-143B] - (A) Representative images of immunohistological staining (brown) of SOX2+ olfactory receptor neurons (ORN) progenitor cells, Gap43+ immature ORNs, Ki67+ proliferating cells & cleaved caspase-3+ (Cas3+) apoptotic cells. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Arrowheads indicate Cas3+ apoptotic cells in the OE (n = 6). (B) Numbers of SOX2+ ORN progenitors & Ki67+ actively proliferating cells per mm of the basal layer, & Gap43+ immature ORNs & Cas3+ apoptotic cells per mm of the OE in saline or CSS-treated mice. Data represent means ± SEM (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001 compared with saline-treated mice (one-way ANOVA). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29950987), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-143b_rabbit-polyclonal-gap-43-antibody-biotin-310202415363728.jpg "Immunohistochemistry: GAP-43 Antibody [Biotin] [NB300-143B] - (A) Representative images of immunohistological staining (brown) of SOX2+ olfactory receptor neurons (ORN) progenitor cells, Gap43+ immature ORNs, Ki67+ proliferating cells & cleaved caspase-3+ (Cas3+) apoptotic cells. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Arrowheads indicate Cas3+ apoptotic cells in the OE (n = 6). (B) Numbers of SOX2+ ORN progenitors & Ki67+ actively proliferating cells per mm of the basal layer, & Gap43+ immature ORNs & Cas3+ apoptotic cells per mm of the OE in saline or CSS-treated mice. Data represent means ± SEM (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001 compared with saline-treated mice (one-way ANOVA). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29950987), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
