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GAMT Antibody - BSA Free

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Biological Strategies: Western Blot: GAMT Antibody [NBP2-34854] - Detection of Human GAMT by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells.
Immunoprecipitation: GAMT Antibody [NBP2-34854] - Detection of Human GAMT by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

GAMT Antibody - BSA Free Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Guanidinoacetate N-methyltransferase.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAMT
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:1000 - 1:5000
Application Notes
Validation by IP/Western Blot was performed using a Western Blot Gel 4-20%, 10 x 10 cm.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GAMT Antibody - BSA Free

  • EC 2.1.1.2
  • guanidinoacetate N-methyltransferase
  • PIG2
  • TP53I2

Background

GAMT is encoded by this gene is a methyltransferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor. Defects in this gene have been implicated in neurologic syndromes and muscular hypotonia, probably due to creatine deficiency and accumulation of guanidinoacetate in the brain of affected individuals. Two transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GAMT
Entrez
Uniprot