![Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Western Blot Analysis of JEG-3 and K562 cell lysate using Galectin-1 antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Western-Blot-NBP2-53212-img0006.jpg "Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Western Blot Analysis of JEG-3 and K562 cell lysate using Galectin-1 antibody (GAL1/1831).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, MA |
| Clone | GAL1/1831 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54457) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment (around aa12-108) of human Galectin-1 protein (exact sequence is proprietary) (Uniprot: P09382) |
| Localization | Secreted (extracellular matrix) |
| Isotype | IgG1 Lambda |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LGALS1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA. Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Galectin-1 Antibody (NBP2-53212)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Confocal immunofluorescence image of HeLa cells using Galectin-1 Antibody (GAL1/1831). Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Immunocytochemistry-Immunofluorescence-NBP2-53212-img0003.jpg "Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Confocal immunofluorescence image of HeLa cells using Galectin-1 Antibody (GAL1/1831). Green (CF488) and Red Dot is used to label the nuclei Red.")
![Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Formalin-fixed, paraffin-embedded Human Prostate Carcinoma stained with Galectin-1 Monoclonal Antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Immunohistochemistry-Paraffin-NBP2-53212-img0001.jpg "Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Formalin-fixed, paraffin-embedded Human Prostate Carcinoma stained with Galectin-1 Monoclonal Antibody (GAL1/1831).")
![Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Western Blot of HeLa, A431, K562 and 293 Cell Lysates using Galectin-1 Monoclonal Antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Western-Blot-NBP2-53212-img0002.jpg "Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Western Blot of HeLa, A431, K562 and 293 Cell Lysates using Galectin-1 Monoclonal Antibody (GAL1/1831).")
![Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Western Blot Analysis of human HeLa cell lysate using Galectin-1 Antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Western-Blot-NBP2-53212-img0005.jpg "Western Blot: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Western Blot Analysis of human HeLa cell lysate using Galectin-1 Antibody (GAL1/1831).")
![Protein Array: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Galectin-1 Antibody (GAL1/1831). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Protein-Array-NBP2-53212-img0004.jpg "Protein Array: Galectin-1 Antibody (GAL1/1831) [NBP2-53212] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Galectin-1 Antibody (GAL1/1831). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
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