![Western Blot: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Analysis of LGALS1 expression in transfected 293T cell line by LGALS1 monoclonal antibody (M01), clone 1E8-1B2.Lane 1: LGALS1 transfected lysate(14.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-1E8-1B2-Western-Blot-H00003956-M01-img0012.jpg "Western Blot: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Analysis of LGALS1 expression in transfected 293T cell line by LGALS1 monoclonal antibody (M01), clone 1E8-1B2.Lane 1: LGALS1 transfected lysate(14.7 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clone | 1E8-1B2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | LGALS1 (AAH01693, 1 a.a. ~ 135 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD |
| Specificity | LGALS1 - lectin, galactoside-binding, soluble, 1 (galectin 1) |
| Isotype | IgG2b Lambda |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LGALS1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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| Publications using H00003956-M01 | Applications | Species |
|---|---|---|
| Ose R, Ohara O, Nagase T. et al. Galectin-1 and Galectin-3 Mediate Protocadherin-24-Dependent Membrane Localization of ?-catenin in Colon Cancer Cell Line HCT116. Current Chemical Genomics 2012-09-20 [PMID: 23115611] | ||
| Tan HT, Tan S, Lin Q et al. Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells. Mol Cell Proteomics 2008-03-14 [PMID: 18344232] | ||
| Minafra IP, Musso GDCR, Valentino GPB. Proteomic Profiling of In-Vitro Bone-Conditioned Skbr3 Breast Cancer Cells. Journal of Proteomics & Bioinformatics. 2016-03-14 (WB, Human) | WB | Human |
| Pucci-Minafra I, Di Cara G, Musso R et al. Retrospective Proteomic Screening of 100 Breast Cancer Tissues. Proteomes. 2017-07-07 [PMID: 28686225] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Galectin-1 Antibody (H00003956-M01)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Analysis of monoclonal antibody to LGALS1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-1E8-1B2-Immunocytochemistry-Immunofluorescence-H00003956-M01-img0007.jpg "Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Analysis of monoclonal antibody to LGALS1 on HeLa cell. Antibody concentration 10 ug/ml.")
![Western Blot: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - LGALS1 monoclonal antibody (M01), clone 1E8-1B2 Analysis of LGALS1 expression in HepG2.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-1E8-1B2-Western-Blot-H00003956-M01-img0011.jpg "Western Blot: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - LGALS1 monoclonal antibody (M01), clone 1E8-1B2 Analysis of LGALS1 expression in HepG2.")
![Immunoprecipitation: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Analysis of LGALS1 transfected lysate using anti-LGALS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LGALS1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-1E8-1B2-Immunoprecipitation-H00003956-M01-img0008.jpg "Immunoprecipitation: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Analysis of LGALS1 transfected lysate using anti-LGALS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LGALS1 MaxPab rabbit polyclonal antibody.")
![Sandwich ELISA: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Detection limit for recombinant GST tagged LGALS1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-1E8-1B2-Sandwich-ELISA-H00003956-M01-img0009.jpg "Sandwich ELISA: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Detection limit for recombinant GST tagged LGALS1 is 1 ng/ml as a capture antibody.")
![Sandwich ELISA: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Detection limit for recombinant GST tagged LGALS1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-1E8-1B2-Sandwich-ELISA-H00003956-M01-img0010.jpg "Sandwich ELISA: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Detection limit for recombinant GST tagged LGALS1 is 0.03 ng/ml as a capture antibody.")
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