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GAD2/GAD65 Antibody (5D9G12) - BSA Free

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Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis of Hela cells using GAD2 mouse mAb (green) and negative control (red).
Western Blot: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Analysis using GAD2 mAb against human GAD2 (AA: 1-100) recombinant protein. (Expected MW is 36.3 kDa)
ELISA: GAD2/GAD65 Antibody (5D9G12) [NBP2-52471] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, CyTOF-ready
Clone
5D9G12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52471-0.025mg
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GAD2/GAD65 Antibody (5D9G12) - BSA Free Summary

Immunogen
Purified recombinant fragment of human GAD2/GAD65 (AA: 1-100) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GAD2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
65.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for GAD2/GAD65 Antibody (5D9G12) - BSA Free

  • EC 4.1.1
  • EC 4.1.1.15
  • GAD2
  • GAD65
  • GAD-65
  • GAD65MGC161605
  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
  • glutamate decarboxylase 2
  • Glutamate decarboxylase 65 kDa isoform
  • Glutamate decarboxylase-2 (pancreas)
  • MGC161607,65 kDa glutamic acid decarboxylase

Background

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GAD2