![Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAD1-GAD67-Antibody-HL1096-Western-Blot-NBP3-13716-img0001.jpg "Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Ca, Fe, ZeSpecies Glossary |
| Applications | WB, IHC, IHC |
| Clone | HL1096 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GAD1/GAD67. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GAD1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 67 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Simple Western IA-2/PTPRN Antibody (815811) [Unconjugated]](https://images.novusbio.com/images2/IA2_MAB7906_Simple_Western_17659.jpg)
![Immunohistochemistry IA-2/PTPRN Antibody (815811) [Unconjugated]](https://images.novusbio.com/images2/IA2_MAB7906_Immunohistochemistry_12951.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for GAD1/GAD67 Antibody (NBP3-13716)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GAD1 |
![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - GAD1/GAD67 antibody [HL1096] detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAD1/GAD67 stained by GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GAD1-GAD67-Antibody-HL1096-Immunohistochemistry-Paraffin-NBP3-13716-img0004.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - GAD1/GAD67 antibody [HL1096] detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAD1/GAD67 stained by GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - GAD1/GAD67 antibody [HL1096] detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: GAD1/GAD67 stained by GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a nerual marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GAD1-GAD67-Antibody-HL1096-Immunohistochemistry-Paraffin-NBP3-13716-img0002.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - GAD1/GAD67 antibody [HL1096] detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: GAD1/GAD67 stained by GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a nerual marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - GAD1/GAD67 antibody [HL1096] detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GAD1/GAD67 stained by GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GAD1-GAD67-Antibody-HL1096-Immunohistochemistry-Paraffin-NBP3-13716-img0003.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - GAD1/GAD67 antibody [HL1096] detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GAD1/GAD67 stained by GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![IHC-Wm- GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.Green: GAD67 stained by GAD67 antibody [HL1096] diluted at 1:200.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC](http://images.novusbio.com/fullsize/nbp3-13716_rabbit-gad1-gad67-mab-hl1096-immunohistochemistry-whole-mount-304202411206..jpg "IHC-Wm- GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.Green: GAD67 stained by GAD67 antibody [HL1096] diluted at 1:200.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC")
![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - Dog cerebellum.GAD67 stained by GAD67 antibody [HL1096] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13716_rabbit-gad1-gad67-mab-hl1096-immunohistochemistry-paraffin-3042024112117..jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - Dog cerebellum.GAD67 stained by GAD67 antibody [HL1096] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - Cat cerebellum.GAD67 stained by GAD67 antibody [HL1096] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13716_rabbit-gad1-gad67-mab-hl1096-immunohistochemistry-paraffin-3042024112219..jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - Cat cerebellum.GAD67 stained by GAD67 antibody [HL1096] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -Human tissue extract (10 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13716_rabbit-gad1-gad67-mab-hl1096-western-blot-3042024112412..jpg "Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -Human tissue extract (10 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13716_rabbit-gad1-gad67-mab-hl1096-western-blot-3042024112539..jpg "Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13716_rabbit-gad1-gad67-mab-hl1096-western-blot-304202411275..jpg "Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")