![Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate (50 ug) from HeLa, MCF-7, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP1antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 1 second.](http://images.novusbio.com/fullsize/G3BP1-Antibody-Western-Blot-NBP1-18922-img0008.jpg "Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate (50 ug) from HeLa, MCF-7, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP1antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 1 second.")
| Reactivity | Hu, Mu, Ma, Pm-OrSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human GTPase activating protein (SH3 domain) binding protein 1 using the numbering given in entry NP_005745.1 (GeneID 10146). |
| Predicted Species | Orangutan (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | G3BP1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Publications using NBP1-18922 | Applications | Species |
|---|---|---|
| Choudhury P, Bussiere L, Miller CL. Mammalian Orthoreovirus Factories Modulate Stress Granule Protein Localization by Interaction with G3BP1. J. Virol. 2017-08-09 [PMID: 28794026] (Mammal) | Mammal | |
| Fujimura K, Sasaki AT, Anderson P. Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules. Nucleic Acids Res. 2012-06-20 [PMID: 22718973] | ||
| Bussiere LD Examination of viral infection and host response to define therapeutic potential of oncolytic mammalian orthoreovirus Thesis 2020-01-01 (ICC/IF, Human) | ICC/IF | Human |
| Honda M, Oki S, Kimura R Et Al. High-depth spatial transcriptome analysis by photo-isolation chemistry Nature communications 2021-07-20 [PMID: 34285220] (IF/IHC) | IF/IHC |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
ICC | Human | 02/12/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: G3BP1 Antibody [NBP1-18922] - G3BP1 in red (Alexa fluor 555) and nucleus in blue (DAPI). Human primary fibroblasts untreated in the left and treated in the right with G3BP1 stress granules labeling. Alexa fluor 555 1s exposure and DAPI 1s exposure. Fixation: PFA 4% 10min RT, permeabilization: Triton 0.1% TBS, blocking: Donkey Serum 10% TBS-T, Ab dilution: 1:1000, Secondary Ab: Alexa fluor 555 Donkey anti-rabbit 1:600, Mounting medium with DAPI. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/G3BP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18922-img0007.jpg "Immunocytochemistry/Immunofluorescence: G3BP1 Antibody [NBP1-18922] - G3BP1 in red (Alexa fluor 555) and nucleus in blue (DAPI). Human primary fibroblasts untreated in the left and treated in the right with G3BP1 stress granules labeling. Alexa fluor 555 1s exposure and DAPI 1s exposure. Fixation: PFA 4% 10min RT, permeabilization: Triton 0.1% TBS, blocking: Donkey Serum 10% TBS-T, Ab dilution: 1:1000, Secondary Ab: Alexa fluor 555 Donkey anti-rabbit 1:600, Mounting medium with DAPI. Image submitted by a verified customer review.")
![Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Affinity purified rabbit anti-G3BP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. G3BP1 was also immunoprecipitated by rabbit anti-G3BP1 antibody NBP1-18923, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/G3BP1-Antibody-Western-Blot-NBP1-18922-img0006.jpg "Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Affinity purified rabbit anti-G3BP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. G3BP1 was also immunoprecipitated by rabbit anti-G3BP1 antibody NBP1-18923, which recognizes a downstream epitope.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
