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G3BP1 [p Ser232] Antibody

Images

 
Western Blot: G3BP1 [p Ser232] Antibody [NB100-81931] - Analysis of extracta from 293 cells untreated or treated with starvation using G3B P-1 Antibody.
Immunocytochemistry/ Immunofluorescence: G3BP1 [p Ser232] Antibody [NB100-81931] - Staining of methanol-fixed Hela cells.
Immunohistochemistry-Paraffin: G3BP1 [p Ser232] Antibody [NB100-81931] - Analysis of Human Breast Carcinoma tissue using NB100-81931 (left) or the same antibody preincubated with blocking peptide (right)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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G3BP1 [p Ser232] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human G3BP1 around the phosphorylation site of serine 232 (S-S-SP-P-A).
Modification
p Ser232
Specificity
Detects endogenous levels of G3BP1 only when phosphorylated at serine 232.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
G3BP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for G3BP1 [p Ser232] Antibody

  • ATP-dependent DNA helicase VIII
  • EC 3.6.1
  • EC 3.6.4.12
  • EC 3.6.4.13
  • G3BP-1
  • G3BPRas-GTPase-activating protein SH3-domain-binding protein
  • GAP binding protein
  • GAP SH3 domain-binding protein 1
  • GTPase activating protein (SH3 domain) binding protein 1
  • hDH VIII
  • HDH-VIII
  • MGC111040
  • ras GTPase-activating protein-binding protein 1
  • RasGAP-associated endoribonuclease G3BP

Background

G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for G3BP1 Antibody (NB100-81931) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on G3BP1.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

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Bioinformatics

Gene Symbol G3BP1
Entrez
Uniprot