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G3BP1 Antibody (S09-5B9)

Images

 
Western Blot: G3BP1 Antibody (S09-5B9) [NBP3-15030] - Western blot detection of G3BP1 in K562, C6, Hela cell lysates using G3BP1 Rabbit mAb (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 68kDa.
Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (S09-5B9) [NBP3-15030] - Immunofluorescence of G3BP1 (green) in Hela cells using G3BP1 Rabbit mAb at dilution 1/200, and DAPI (blue)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
S09-5B9
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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G3BP1 Antibody (S09-5B9) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human G3BP1 (Uniprot#: Q13283)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
G3BP1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1/50-1/200
  • Western Blot 1/2000-1/10000
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for G3BP1 Antibody (S09-5B9)

  • ATP-dependent DNA helicase VIII
  • EC 3.6.1
  • EC 3.6.4.12
  • EC 3.6.4.13
  • G3BP-1
  • G3BPRas-GTPase-activating protein SH3-domain-binding protein
  • GAP binding protein
  • GAP SH3 domain-binding protein 1
  • GTPase activating protein (SH3 domain) binding protein 1
  • hDH VIII
  • HDH-VIII
  • MGC111040
  • ras GTPase-activating protein-binding protein 1
  • RasGAP-associated endoribonuclease G3BP

Background

G3BP1 encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for G3BP1 Antibody (NBP3-15030) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on G3BP1.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

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Bioinformatics

Gene Symbol G3BP1