![Western Blot: Fyn Antibody [NBP1-82685] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Fyn-Antibody-Western-Blot-NBP1-82685-img0010.jpg "Western Blot: Fyn Antibody [NBP1-82685] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: SFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIR |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FYN |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB reported in scientific literature (PMID: 26224309). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Fyn Antibody (NBP1-82685)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FYN |
![Immunocytochemistry/Immunofluorescence: Fyn Antibody [NBP1-82685] - Staining of human cell line AF22 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Fyn-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82685-img0009.jpg "Immunocytochemistry/Immunofluorescence: Fyn Antibody [NBP1-82685] - Staining of human cell line AF22 shows localization to plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] - Staining of human tonsil shows strong cytoplasmic positivity in a subset of reaction center cells and lymphoid cells outside reaction centra.](http://images.novusbio.com/fullsize/Fyn-Antibody-Immunohistochemistry-Paraffin-NBP1-82685-img0002.jpg "Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] - Staining of human tonsil shows strong cytoplasmic positivity in a subset of reaction center cells and lymphoid cells outside reaction centra.")
![Western Blot: Fyn Antibody [NBP1-82685] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/Fyn-Antibody-Western-Blot-NBP1-82685-img0007.jpg "Western Blot: Fyn Antibody [NBP1-82685] - Analysis in human cell line U-87 MG.")
![Western Blot: Fyn Antibody [NBP1-82685] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Fyn-Antibody-Western-Blot-NBP1-82685-img0008.jpg "Western Blot: Fyn Antibody [NBP1-82685] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")