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Fyn Antibody [Unconjugated]

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Fyn was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Goat Anti-Human Fyn Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3574) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Fyn was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Goat Anti-Human Fyn Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3574) at 15 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3574-SP
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Fyn Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Fyn
Met1-Gly83
Accession # P06241
Specificity
Detects human Fyn in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FYN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 10-20 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fyn Antibody [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.2
  • FYN oncogene related to SRC, FGR, YES
  • Fyn
  • MGC45350
  • OKT3-induced calcium influx regulator
  • p59-Fyn
  • protein-tyrosine kinase fyn
  • Proto-oncogene c-Fyn
  • Proto-oncogene Syn
  • proto-oncogene tyrosine-protein kinase fyn
  • SLK
  • SLKc-syn protooncogene
  • src/yes-related novel
  • Src-like kinase
  • SYN
  • tyrosine kinase p59fyn(T)
  • tyrosine-protein kinase Fyn

Background

Fyn is a ubiquitously expressed member of the Src family of intracellular tyrosine kinases. Fyn activity is implicated in T cell receptor signaling, adhesion-mediated signaling, and regulation of brain function. Mice lacking fyn display various deficits, including impaired spatial memory, hypomyelination, and uncoordinated hippocampal structure, suggesting that Fyn is involved in multiple neural signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FYN
Uniprot