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FoxO1/FKHR Antibody (7H3)

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Immunocytochemistry/Immunofluorescence: FOXO1A Antibody (7H3) [NB200-307] - Analysis of paraformaldehyde-fixed HeLa, using antibody (7H3) at 1:100 dilution.
Immunocytochemistry/ Immunofluorescence: FOXO1A Antibody (7H3) [NB200-307] - Analysis of paraformaldehyde-fixed HeLa, using antibody (7H3) at 1:100 dilution.
Immunohistochemistry-Paraffin: FOXO1A Antibody (7H3) [NB200-307] - Analysis of paraffin-embedded Colon ca, using antibody (7H3) at 1:50 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
7H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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FoxO1/FKHR Antibody (7H3) Summary

Immunogen
Amino acids 242-655 of FKHR expressed in E. coli.
Specificity
This is specific for FKHR.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOXO1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 0.1-2 ug/ml
Application Notes
ICC/IF, IHC- Assay dependent
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
FoxO1/FKHR Overexpression Lysate
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB200-307.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29812970).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for FoxO1/FKHR Antibody (7H3)

  • FKH1
  • FKHR
  • FKHRforkhead box protein O1
  • forkhead box O1
  • Forkhead box protein O1A
  • forkhead homolog in rhabdomyosarcoma
  • Forkhead in rhabdomyosarcoma
  • FoxO1
  • FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

NB 200-307 recognizes FKHR (ForKHead in Rhabdomyosarcoma), a 70kDa protein, which is one member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxO1/FKHR Antibody (NB200-307)(1)

We have publications tested in 1 confirmed species: Mouse.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxO1/FKHR Antibody (NB200-307) (0)

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Control Lysate(s)

FoxO1/FKHR Overexpression Lysate
Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FOXO1
Entrez