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FoxD3 Antibody

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Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human FoxD3. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FoxD3 aa 1-140 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2819) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxD3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human FoxD3. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FoxD3 aa 1-140 Antigen ...read more
FoxD3 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human FoxD3 aa 1-140 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2819) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2819-SP
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FoxD3 Antibody Summary

Immunogen
E. coli-derived recombinant human FoxD3
Met1-Val140
Accession # Q9UJU5
Specificity
Detects human FoxD3 (aa 1-140) in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxP3, rhFoxP1, and rhFoxO3A is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
FOXD3
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxD3 Antibody

  • AIS1
  • CWH3
  • forkhead box D3
  • forkhead box protein D3
  • FoxD3
  • Genesis
  • HFH2
  • HNF3/FH transcription factor genesis

Background

FOXD3 (forkhead box protein D3; also HNF3, HFH2 and Genesis) is a 58-60 kDa member of the winged helix transcription factor gene family. It is expressed in the epiblast, migrating neural crest, and neural progenitors of the developing spinal cord. FOXD3 is believed to maintain an uncommitted state in multipotent neural crest stem cells, and to induce cell cycle arrest via p53 and p21cip1. Human FOXD3 is 478 amino acids (aa) in length. It contains a forkhead DNA binding domain (aa 141‑235) plus an Ala-rich region (aa 262-473). Over aa 1-140 and 302-462, human FOXD3 shares 76% and 91% aa identity with mouse FoxD3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FOXD3
Uniprot