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FLI1 Antibody (1F6)

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Western Blot: FLI1 Antibody (1F6) [H00002313-M05] - Analysis of FLI1 expression in transfected 293T cell line by FLI1 monoclonal antibody (M05), clone 1F6. Lane 1: FLI1 transfected lysatE (50.982 KDa). Lane 2: Non-transfected lysate.
Western Blot: FLI1 Antibody (1F6) [H00002313-M05] - Analysis of FLI1 expression in transfected 293T cell line by FLI1 monoclonal antibody (M05), clone 1F6. Lane 1: FLI1 transfected lysatE (50.982 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: FLI1 Antibody (1F6) [H00002313-M05] - Analysis of monoclonal antibody to FLI1 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: FLI1 Antibody (1F6) [H00002313-M05] - Western blot analysis of FLI1 over-expressed 293 cell line, cotransfected with FLI1 Validated Chimera RNAi or non-transfected control. Blot probed with H00002313-M05. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, KD
Clone
1F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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FLI1 Antibody (1F6) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
FLI1 (AAH01670, 1 a.a. ~ 452 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDGTIKEALSVVSDGQSLFDSAYGAAAHLPKADMAASGSPDYGQPHKINPLPPQQEWISQPVRVNVKREYDHMNGSRESPVDCSVSKCSKLVGGGESNPMNYNSYMDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQWLEWAIKEYSLMEIDTSFFQNMDGKELCKMNKEDFLRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQSSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERISKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQQKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY
Specificity
FLI1 (1F6)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FLI1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FLI1 Antibody (1F6)

  • ERGB
  • EWSR2
  • FLI1
  • Friend leukemia integration 1 transcription factor
  • Friend leukemia virus integration 1
  • Proto-oncogene Fli-1
  • SIC-1
  • Transcription factor ERGB

Background

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2 Erg-1, Erg-2, Elk, E74, Fli-1, PU.1 and PEA3. Members of the Ets gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FLI1
Entrez
OMIM
Uniprot