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FKBP12 Antibody (OTI3B3)

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Western Blot: FKBP12 Antibody (3B3) [NBP2-02093] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP12.
Western Blot: FKBP12 Antibody (3B3) [NBP2-02093] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: FKBP12 Antibody (3B3) [NBP2-02093] Staining of paraffin-embedded Human Kidney tissue using anti-FKBP12 mouse monoclonal antibody.
Flow Cytometry: FKBP12 Antibody (3B3) [NBP2-02093] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FKBP12 antibody, and then ...read more
Immunohistochemistry-Paraffin: FKBP12 Antibody (3B3) [NBP2-02093] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FKBP12 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI3B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70741AF350
Alexa Fluor 405
NBP2-70741AF405
Alexa Fluor 488
NBP2-70741AF488
Alexa Fluor 532
NBP2-70741AF532
Alexa Fluor 594
NBP2-70741AF594
Alexa Fluor 647
NBP2-70741AF647
Alexa Fluor 700
NBP2-70741AF700
Alexa Fluor 750
NBP2-70741AF750
Allophycocyanin
NBP2-70741APC
Allophycocyanin/Cy7
NBP2-70741APCCY7
Biotin
NBP2-70741B
CoraFluor 1
NBP2-70741CL1
DyLight 350
NBP2-70741UV
DyLight 405
NBP2-70741V
DyLight 488
NBP2-70741G
DyLight 550
NBP2-70741R
DyLight 594
NBP2-70741DL594
DyLight 650
NBP2-70741C
DyLight 680
NBP2-70741FR
DyLight 755
NBP2-70741IR
FITC
NBP2-70741F
HRP
NBP2-70741H
Janelia Fluor 525
NBP2-70741JF525
Janelia Fluor 549
NBP2-70741JF549
Janelia Fluor 585
NBP2-70741JF585
Janelia Fluor 635
NBP2-70741JF635
Janelia Fluor 646
NBP2-70741JF646
Janelia Fluor 669
NBP2-70741JF669
PE
NBP2-70741PE
PE/Atto594
NBP2-70741PEATT594
PE/Cy5.5
NBP2-70741PECY55
PE/Cy7
NBP2-70741PECY7
PerCP
NBP2-70741PCP
mFluor Violet 450 SE
NBP2-70741MFV450
mFluor Violet 500 SE
NBP2-70741MFV500
mFluor Violet 610 SE
NBP2-70741MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-70741
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FKBP12 Antibody (OTI3B3) Summary

Immunogen
Full length human recombinant protein of human FKBP1A (NP_000792) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
FKBP1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200-1:1000
Theoretical MW
11.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FKBP12 Antibody (OTI3B3)

  • 12 kDa FKBP
  • EC 5.2.1.8
  • FK506 binding protein 1A, 12kDa
  • FK506-binding protein 1A
  • FKBP1
  • FKBP12
  • FKBP12C
  • FKBP12FKBP12-Exip3
  • FKBP-12T-cell, 12-kD
  • FKBP1a
  • FKBP-1A
  • peptidyl-prolyl cis-trans isomerase FKBP1A
  • PKC12
  • PPIase FKBP1A
  • PPIASE
  • protein kinase C inhibitor 2
  • rotamase

Background

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin Ryanodine Receptor Antibody (34C) - BSA Free
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Antibody (787924)
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FKBP12 Antibody
(OTI3B3)
NBP2-02093
FKBP12 Antibody (OTI3B3)
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC

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Bioinformatics

Gene Symbol FKBP1A
Entrez